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Ç¥ 2. Å丶Åä multiplex PCR ¹× Nested-PCR primer setÀÇ ¿°±â¼¿ ºÐ¼®(°è¼Ó)
PepMV
ACTTCCTTGCTGTTCGGAAGTTGCACAAGTTACACCAAGGAGAAGCAATGAGACTTGGTGAAAAGAGCCCTC
CAAAAGCAACACATTGAGGGGTTAAGTTTTCCCCAGTTCGAAATGGAAAGATCAACTCTGATTAATTTACTTC
AATTGCACCACTTCGAGCCAAAACTCAGTGTTGAAGGAATCATAGTTGTGCACGGAATTGCAGGCACTGGGA
AAACCACTTTACTTAGGACTTTATTTTCTGCTTACCCTAGCTTAGTTATAGGTTCACCTAGGCCTTGCTATTTAG
ATAAACAAAACAAAATTTCACAAGTTTGCTTATCTTGCTTTCCCAATACCCATTGTGATATTGTCGATGAGTAT
CATTTGCTAGAAAGTTTTCCAGAACCAAAATTGGCTATCTTTGGTGACCCCTGTCAATGCACATACATTGAGA
GACTTAGAGTCCCACATTACACTTCCTTCAGAACTCATAGATTTGGAAAGTCAACTGCTGAGATTTTGAACAA
ACTGTTTGACCTTAATATAGTCTCAGTTAAGAAAGAAGACGACATCGTTGAATTCTTTAACCCTTTCGAAGTTG
ACCCCACTGAGCATATCTCTGCCTCTGAAGAAGAAGTCTTGGACTTTGTTTCTGACCAAGTGGTGACCACTAG
CTCAGAGGAACTAGCAGGACTTGAGTTTGCAGAAACAACTTTCTACTGCACAACATTGGCCGCAGCTGTTGC
TGAAAATCCTGCTAAGACTTTCATCTCTCTGACTAGACACACCCACAAACTCACCATTG[
CAGGT>]CTAACTCCTAGAGCTGACCTCACTGACACATACAAAATCATTGCCATTGCTTTCTTGTTGTCAGCTT
GCATTTACTTCCAAAATAGCCACTACCAACCTGTTGCTGGAGACAACTTGCACCGTTTGCCTTTTGGTGGCCA
ATATCAAGACGGCACCAAAAAGATATCTTATTTTCCACAACAGCAGTCATACTTTCATTCTGGAAACAAATTA
AATGTCCTCATACTTATCTTCATTCTCACATTTAAATTTAGTTTTAGCTTTAGTCG
TACTACTCACCAGCATTCTTGCTATAACACACATTCA[GCAACCAACAATACACAACC]
[ ], multiplex RT-PCR primer binding site; < >, multiplex Nested-PCR primer binding site
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Å丶Åä multiplex RT&Nested-PCR primer set¸¦ °³¹ßÇÏ¿´À¸¸ç(Ç¥ 3), ±âÁ¸ÀÇ monoplex¿Í
ºñ±³ÇÏ´õ¶óµµ °ËÃâ ÇÑ°è ¸é¿¡¼ Å« Â÷ÀÌ°¡ ¾ø´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù (Ç¥ 4).
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PepMV
ACTTCCTTGCTGTTCGGAAGTTGCACAAGTTACACCAAGGAGAAGCAATGAGACTTGGTGAAAAGAGCCCTC
CAAAAGCAACACATTGAGGGGTTAAGTTTTCCCCAGTTCGAAATGGAAAGATCAACTCTGATTAATTTACTTC
AATTGCACCACTTCGAGCCAAAACTCAGTGTTGAAGGAATCATAGTTGTGCACGGAATTGCAGGCACTGGGA
AAACCACTTTACTTAGGACTTTATTTTCTGCTTACCCTAGCTTAGTTATAGGTTCACCTAGGCCTTGCTATTTAG
ATAAACAAAACAAAATTTCACAAGTTTGCTTATCTTGCTTTCCCAATACCCATTGTGATATTGTCGATGAGTAT
CATTTGCTAGAAAGTTTTCCAGAACCAAAATTGGCTATCTTTGGTGACCCCTGTCAATGCACATACATTGAGA
GACTTAGAGTCCCACATTACACTTCCTTCAGAACTCATAGATTTGGAAAGTCAACTGCTGAGATTTTGAACAA
ACTGTTTGACCTTAATATAGTCTCAGTTAAGAAAGAAGACGACATCGTTGAATTCTTTAACCCTTTCGAAGTTG
ACCCCACTGAGCATATCTCTGCCTCTGAAGAAGAAGTCTTGGACTTTGTTTCTGACCAAGTGGTGACCACTAG
CTCAGAGGAACTAGCAGGACTTGAGTTTGCAGAAACAACTTTCTACTGCACAACATTGGCCGCAGCTGTTGC
TGAAAATCCTGCTAAGACTTTCATCTCTCTGACTAGACACACCCACAAACTCACCATTG[
CAGGT>]CTAACTCCTAGAGCTGACCTCACTGACACATACAAAATCATTGCCATTGCTTTCTTGTTGTCAGCTT
GCATTTACTTCCAAAATAGCCACTACCAACCTGTTGCTGGAGACAACTTGCACCGTTTGCCTTTTGGTGGCCA
ATATCAAGACGGCACCAAAAAGATATCTTATTTTCCACAACAGCAGTCATACTTTCATTCTGGAAACAAATTA
AATGTCCTCATACTTATCTTCATTCTCACATTTAAATTTAGTTTTAGCTTTAGTCG
TACTACTCACCAGCATTCTTGCTATAACACACATTCA[GCAACCAACAATACACAACC]
[ ], multiplex RT-PCR primer binding site; < >, multiplex Nested-PCR primer binding site
°á·Ð
Å丶Åä Á¾ÀÚ¿¡ °¨¿°µÉ ¼ö ÀÖ´Â ArMV, TBRV, ToRSV ¹× PepMV¸¦ ÇÑ ¹ø¿¡ °Ë»çÇÒ ¼ö ÀÖ´Â
Å丶Åä multiplex RT&Nested-PCR primer set¸¦ °³¹ßÇÏ¿´À¸¸ç(Ç¥ 3), ±âÁ¸ÀÇ monoplex¿Í
ºñ±³ÇÏ´õ¶óµµ °ËÃâ ÇÑ°è ¸é¿¡¼ Å« Â÷ÀÌ°¡ ¾ø´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù (Ç¥ 4).
½Ä¹°°Ë¿ª¹ÙÀÌ·¯½º °Ë»ç¹ý ? 497
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