총 508페이지

RT&Nested-PCR 검사법
[GACGATTCCCTCCGGTAGTTATGC]AGACGTGTATAATGCGCGCAACATGACACGCGTTTTCCGCCCACAATCT
GTACAGGGTTCTTCTTTGGCGGAAGCGCAATTTAATGAATCGCACGCTGTGAATATGCTAGTGCGAATTGATTT
ACCTGATGGCAACATTATTTCTGCCTGCAGGTTTCGCGGGAAGTCTTTGGCTTTGACTAAACATCAGGCCTTAA
CCATACCGCCAGGTGCTAAAATCCATATTGTATATACTGACAACAATGGAAATACTAAAGCACCTCTGACCCAT
TTTTTCCAACCTACTGGACCTAATGGAGAACGTTTTTTGAGATTCTTCAACGGGACAGAGGTTTGTATTTATTCT
CACCCTCAACTTTCAGCTTTGCCTGGCGCTCCACAAAACTATTTTTTGAAAGATGTGGAGAAAATATCTGGTGA
CATAGCCATTAAAGGTTGTGGCATCAAGCTAGGTAGAACTAGCGTTGGCGAGTGTGTTGGTGTTAAGGACAAT
GAACCCGTCTTAAATCACTGGCGCGCTGTCGCGAAGGTTCGCACCACCAAGATCACTATCGACAATTATGCAG
AGGGTGGTGATTATTCCAATGATCTTCCTACGTCCATCATCTCTGAGTATGTAAATTCACCAGAAGATTGTGGC
GCGCTTTTAGTCGCCCATCTTGAAGGTGGTTACAAAATCATAGGGATGCACGTGGCGGGATCTTCTTATTCCGT
CGAGGTTGATGGAGTGCAGATGCCAAGATATATATCTCATGCCTCCTTCTTCCCCGATTATTCTTCTTTTGCTCC
TTGCCAGTCTAGTGTCATCAAGTCTCTAATTCAAGAGGCTGGCGTTGAGGAGCGTGGGGTTTCTAAAGTGGGA
CATATTAAAGATCCTGCTGAAACGCCCCATGTTGGAGGGAAAACTAAGCTTGAATTGGTTGATGAAGCCTTCTT
GGTGCCATCACCAGTTGAGGTAAAGATTCCCTCCATTTTATCTAAAGATGATCCGCGCATTCCTGAAGCGTACA
AGGGCTATGATCCACTGGGCGATGCCATGGAGAAGTTTTATGAGCCCATGTTGGATCTGGATGAAGATGTCTT
GGAGAGCGTTATGGCAGATATGTATGATGAATTCTATGATTGCCAAACGACTCTTCGCATTATGTCTGATGACG
AAGTTATCAATGGCAGCGATTTTGGTTTTAATATTGAAGCCGTTGTCAAAGGTACTTCTGAAGGCTACCCGTTC
GTTCTGAGTCGGCGACCGGGTGAGAAGGGCAAAGCTCGCTTTTTAGAAGAGCTTGAACCCCAACCAGGTGAC
ACTAAGCCCAAATATAAACTAGTCGTGGGCACTGAGGTGCATTCTGCTATGGTGGCGATGGAACAACAGGCGC
GTACTGAAGTTCCTTTGCTTATTGGCATGGATGTTCCAAAGGACGAGAGACTCAAACCGTCTAAGGTGTTGGAG
AAGCCGAAGACGCGTACATTCGTTGTTCTCCCAATGCACTATAACTTGCTGCTGCGTTAGTATGTGGGAATTTT
GTGTTCTAGCATGCAAGTTAATAGGCATCGTCTAGCATGTGCTGTGGGCACCAACCCATATTCACGTGATTGGA
CGGACATTTATCAGCGCCTGGCTGAGAAAAATTCAGTGGCCTTGAATTGTGATTATAGTCGCTTTGATGGGCTC
CTCAATTACCAGGCATATGTGCATATTGTTAATTTTATTAATAAATTGTACAACGATGAACATTCTATCGTGCGT
GGCAATCTTTTGATGGCTATGTATGGTAGGTGGAGTGTGTGTGGGCAGAGAGTTTTCGAAGTCCGCGCTGGCA
TGCCCTCTGGGTGTGCGCTCACCGTGATCATCAATTCACTTTTTAACGAGATGTTGATCAGGTATGTTTATCGCA
TCACCGTACCACGCCCCCTTGTAAATAATTTTAAACAGGAGGTGTGTTTGATTGTTTATGGTGATGATAATTTAA
TTTCTATTAAGCCGGACACCATGAAATATTTTAATGGTGAGCAAATTAAAACCATTCTGGCTAAATATAAAGTTA
CCATTACTGATGGCAGTGATAAGAACTCACCTGTTCTTAGAGCCAAACCCTTGAAACAGCTCGATTTTTTGAAG
AGAGGTTTCAGGGTTGAAAGTGATGGGAGGGTGCTTGCCCCTTTAGATTTGCAAGCTATCTATTCTTCCCTGTA
TTATATTAATCCGCAGGGAAATATATTAAATTCTTTGTTTTTGAATGCTCGAGTCGCTTTGAGAGAGTTATATCTC
CATGGCGATGTTGAGCAATTTACTGCTGTCAGGAATTTTTACGTCAAGCAAATTGGCGGAAATTTCTTGAGTCT
ACCCCAGTGGAGGCACTGCGCTTCGTTCCATGATGAACAATATTCTCAGTGGAAGCCGTGGTCCCCCGTTAAA
TTCTTGGAGGTA[GATGTGCCCGATGCTAAATTTT]
그림 12. ToRSV positive control (N10 / C20, 2,474 bp)의 염기서열
[ ], Primer binding site
[TACCACGCCCCCTTGTA]AGTAATTTTAAACAGGAGGTGTGTTTGATTGTTTATGGTGATGATAATTT
ATTAAGCCGGACACCA>TGAAATATTTTAATGGTGAGCAAATTAAAACCATTCTGGCTAAATATAAAGTTACCAT
TACTGATGGCAGTGATAAGAACTCACCTGTTCTTAGAGCCAAACCCTTGAAACAGCTCGATTTTTTGAAGAGAG
GTTTCAGGGTTGAAAGT{GGATCC}GATGGGAGGGTGCTTGCCCCTTTAGATTTGCAAGCTATCTATTCTTCCCT
GTATTATATTAATCCGCAGGGAAATATATTAAATTCTTTGTTTTTGAATGCTCGAGTCGCTTTGAGAGAGTTATAT
CTCCATGGCGATGTTGAGCAATTTACTGCTGTCAGGAATTTTTACGTCAAGCAAATTGGCGGAAATTTCTTGAG
TCTACCCCAGTGGAGGCACTGCGCTTCGTTCCATGATGAACAATACCCGT
TAAATTCTTGGAGGTA[GATGTGCCCGATGCTAAATTTT]
그림 13. ToRSV 돌연변이­양성대조구 (N120 / C20, 555 bp)의 염기서열
[ ], Primer binding site; < >, Nested primer binding site; { }, 염기서열 삽입
396 ?

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