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Multiplex RT&Nested-PCR 검사법
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X. 영문초록
1.
Multiplex Nested Reverse Transcription-Polymerase Chain
Title
Reaction Assay for Detection of SqMV, CGMMV and
KGMMV in Cucurbitaceae
Researcher
Siwon Lee, Eun-Ha Kang, Sunhoo Kim and Yong-Gil Shin
The inspection quantity associated with Cucurbitaceae is large portion of
total seed-born quarantine viruses in seed imported period of Korea,
especially Squach mosaic virus(SqMV), Kyuri green mottle mosaic
virus(KGMMV) and Cucumber green mottle mosaic virus(CGMMV).
Therefore, we have developed multiplex primer sets of Cucurbitaceae to
reduce the burden of heavy workload. The sizes of amplifications are
620, 525 and 447 base pair(bp) using the primers and 567, 407 and 301
bp using the nested primers for the genes of SqMV, CGMMV and
KGMMV. Moreover to employ had been using primer set in quarantine
make minimize confusion in changed system. We hope that this multiplex
assay could help quarantine workload effectively in seed imported period.
Keyword
Multiplex nested, Cucurbitaceae, SqMV, KGMMV, CGMMV
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