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Multiplex RT&Nested-PCR °Ë»ç¹ý
Ç¥ 3. Å丶Åä multiplex RT- ¹× PCR primer set
Multiplex RT-PCR
Concentration
Size
Forward
Reverse
[pmol]
[bp]
ArM_N30
ArM_C29
20
780
AACGCGGGGTCTTGCTGGTA
GACCCCGTTCCATTCACTA
TBR_N20
TBR_C70
20
662
GCAGTCTGTTCACCTGCG
AGATGCACGGGGAGATAACT
ToRS_R1_N120
ToRS_R1_C20
30
549
TACCACGCCCCCTTGTA
AAAATTTARCATCGGGCACATC
PepMV_N70
PepMV_C30
20
362
GGGAACTAAATGCCAGGT
GGTTGTGTATTGTTGGTTGC
N-primer [pmol]
3
Multiplex Nested-PCR
Concentration
Size
Forward
Reverse
[pmol]
[bp]
ArM_N22
ArM_C28
5
733
CTGTGCAGGTGCCTAAAGATTG
ACTCTTAGGACTATGCCTAAGT
TBR_N20
TBRN_C70
25
585
GCAGTCTGTTCACCTGCG
TTCCATCCCGCTTCAGCAAGT
ToRS_R1_N130
ToRS_R1_C30
5
439
AATTTCTATTAAGCCGGACACCA
AGACCACGGCTTCCACTGAGAA
PepMV_N70
PepMV_C40
30
281
GGGAACTAAATGCCAGGT
TTGGTGAGGACAATACCC
N-primer [pmol]
-
Ç¥ 4. Å丶Åä monoplex ¹× multiplex °ËÃâ ÇÑ°è ºñ±³
Monoplex end-point
Multiplex end-point
Virus name
RT-PCR
Nested-PCR
RT-PCR
Nested-PCR
-5
-7
-6
-7
ArMV
10
10
10
10
-4
-6
-6
-6
TBRV
10
10
10
10
-5
-5
-5
-7
ToRSV
10
10
10
10
-6
-6
-5
-5
PepMV
10
10
10
10
498 ?
Ç¥ 3. Å丶Åä multiplex RT- ¹× PCR primer set
Multiplex RT-PCR
Concentration
Size
Forward
Reverse
[pmol]
[bp]
ArM_N30
ArM_C29
20
780
AACGCGGGGTCTTGCTGGTA
GACCCCGTTCCATTCACTA
TBR_N20
TBR_C70
20
662
GCAGTCTGTTCACCTGCG
AGATGCACGGGGAGATAACT
ToRS_R1_N120
ToRS_R1_C20
30
549
TACCACGCCCCCTTGTA
AAAATTTARCATCGGGCACATC
PepMV_N70
PepMV_C30
20
362
GGGAACTAAATGCCAGGT
GGTTGTGTATTGTTGGTTGC
N-primer [pmol]
3
Multiplex Nested-PCR
Concentration
Size
Forward
Reverse
[pmol]
[bp]
ArM_N22
ArM_C28
5
733
CTGTGCAGGTGCCTAAAGATTG
ACTCTTAGGACTATGCCTAAGT
TBR_N20
TBRN_C70
25
585
GCAGTCTGTTCACCTGCG
TTCCATCCCGCTTCAGCAAGT
ToRS_R1_N130
ToRS_R1_C30
5
439
AATTTCTATTAAGCCGGACACCA
AGACCACGGCTTCCACTGAGAA
PepMV_N70
PepMV_C40
30
281
GGGAACTAAATGCCAGGT
TTGGTGAGGACAATACCC
N-primer [pmol]
-
Ç¥ 4. Å丶Åä monoplex ¹× multiplex °ËÃâ ÇÑ°è ºñ±³
Monoplex end-point
Multiplex end-point
Virus name
RT-PCR
Nested-PCR
RT-PCR
Nested-PCR
-5
-7
-6
-7
ArMV
10
10
10
10
-4
-6
-6
-6
TBRV
10
10
10
10
-5
-5
-5
-7
ToRSV
10
10
10
10
-6
-6
-5
-5
PepMV
10
10
10
10
498 ?
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