총 147페이지

Collectionandspecimenpreparation
Collection
Whenwhitefliesarecollected,thepupariaareusuallybestcollectedwhilestillattached
to leaf tissues. However, collectors may wish to obtain all stages including the adult.
Sometimes,adultscanbeobtained(reared)frompupae,ifthepupaearecollectedatthe
correct time. Either the whole leaf may be excised and placed in small envelopes or
zip-lock bags to be kept dry until required for preparation or pieces of leaf with the
puparia attached may be placed and stored in >80% ethanol prior to treatment. The
identityofthehostplantandcountryoforiginshouldbenoted.
Althoughadultsareoftenfoundduringtheinspectionofimportedplantsinquarantine
sites,itmaybedifficult(anddestructive)tocapturethemwithhandsorwithatoolsuchas
forceps,giventhatadultsaresmallandactivefliers.Onewaytocollectadultwhitefliesis
toaspiratethemfromtheleaf,butifanaspiratorisnotavailable,anotherwayistoplacea
zip?lockbagovertheleaf,shaketheleafinsidethebag,placethebaginafreezerforafew
minutestoknockthewhitefliesdown,andthenextractthemfromthebag.
Specimenpreparation
Thebestmountsareusuallyobtainedfromthepupariafromwhichadultshaverecently
emerged.However,thepupariafromwhichwaspparasiteshaveemergedaredamagedby
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Collectionandspecimenpreparation

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