총 2151페이지

〈시험 2〉
연
1) 동 물
구
체중 8∼10㎏(4∼6주령)의 돼지 생식기호흡기증후군바이러스에 대한 항체음성 돼지 4마리를
성
사용한다.
과
2) 시 험
활
2마리의 돼지는 접종군, 2마리는 대조군으로 둔다. 접종군의 돼지 2마리에 대하여 백신을
용
1두분씩 각각 근육접종하고 3주후에 2차 접종을 실시한다. 2차 접종 4주후에 대조군과 동시에
집
채혈을 한다. 혈청중화시험은 의 중화 시험방법과 동일하게 실시한다.
3) 판 정
접종군에 대한 돼지 생식기호흡기증후군 바이러스의 각 백신주에 대한 중화 항체가는 4배
이상이어야 하며, 대조군은 음성이어야 한다.
아. 검정의 반복
??가??항에서 ??사??항까지의 시험에서 그 결과를 판정하기 곤란할 때는 해당시험을 반복한다.
[별첨] 세포유지용 배양액(1,000㎖)
α-MEM·········································································································950㎖
Fetal bovine serum·······················································································30㎖
DEAE-dextran···························································································20㎍/㎖
Trehalose·································································································10㎎/㎖
7.5% NaHCO3 ································································································20㎖
Amphotericin B···················································································0.025㎍/㎖
Penicillin G-Na·····················································································100unit/㎖
Streptomycin···························································································100㎍/㎖
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