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gave rise to duct like structure with a well-defined lumen and circumscribed by neatly aligned
cells.
We demonstrated the pluripotency of ES cells into mouse hepatic progenitor cells from NVRQS
ES cells. ES-HPCs expressed liver specific proteins and were able to differentiate into hepatocyte
and bile duct cells. The HPCs established by the present study will be used as a novel in vitro
model for hepatotoxicity evaluation.
It was general tool to use hematopoietic cells originated from bone marrow, when we studid and
treated hematopoietic diseases like leukemia. but, in these days, Study for hematopoietic disease is
focused on hematopoietic stem cell from embryonic stem cell to avoid ethical problem relating
experimental animals and diversity of individual gene. So, we try to establish hematopoietic stem
cell originated from ES cell to use hematopoietic toxicity screening test for livestock.
Mouse ES cells were cultured on the OP9 feeder layer for 4 days and transferred to 2nd
differentiation medium for 21days. after differentiation, ES-derived hematopoietic cells revealed
single round morphology compared to multiple layer clump of ES cell and expressed strongly
CD-117, marker protein of hematopoietic cell.
On the basis of these results, we can assure successful establishment of ES-derived hematopoietic
cells.
ES-derived neural cells can be used as resorces relating to neuronal disease and neurotoxicity
study. So, we establish the neural cell line and maintenance methods for the livestock-derived
neurotoxicity test.
To establish neural progenitor cell line, we induced embryoid body (EB) which were cultured EB
medium for 5 days, and EBs were incubated in ITSF medium for 8 days. Ｗe can observe Cells
maintained a round colony condition and the circumferential department cells. Cytokeratin8 and
Nestin which were neural progenitor cell marker were also observed under fleuroscence
microscope.
Maintenance and characterization method of neural progenitor cells were established but, not
neural cells. For the further study, we will progress differentiation study to neural cell in second
project, "Development and application of embryonic stem cell models for the evaluation of
chemical toxicity." We expect each established stem cell lines and differentiation cell lines can be
important materials for wide range of biotechnology research, toxicity screening test and disease
model development.
Keyword
Embryonic stem cell, EC cell, EG cell, embryoid body, differentiated cell
cells.
We demonstrated the pluripotency of ES cells into mouse hepatic progenitor cells from NVRQS
ES cells. ES-HPCs expressed liver specific proteins and were able to differentiate into hepatocyte
and bile duct cells. The HPCs established by the present study will be used as a novel in vitro
model for hepatotoxicity evaluation.
It was general tool to use hematopoietic cells originated from bone marrow, when we studid and
treated hematopoietic diseases like leukemia. but, in these days, Study for hematopoietic disease is
focused on hematopoietic stem cell from embryonic stem cell to avoid ethical problem relating
experimental animals and diversity of individual gene. So, we try to establish hematopoietic stem
cell originated from ES cell to use hematopoietic toxicity screening test for livestock.
Mouse ES cells were cultured on the OP9 feeder layer for 4 days and transferred to 2nd
differentiation medium for 21days. after differentiation, ES-derived hematopoietic cells revealed
single round morphology compared to multiple layer clump of ES cell and expressed strongly
CD-117, marker protein of hematopoietic cell.
On the basis of these results, we can assure successful establishment of ES-derived hematopoietic
cells.
ES-derived neural cells can be used as resorces relating to neuronal disease and neurotoxicity
study. So, we establish the neural cell line and maintenance methods for the livestock-derived
neurotoxicity test.
To establish neural progenitor cell line, we induced embryoid body (EB) which were cultured EB
medium for 5 days, and EBs were incubated in ITSF medium for 8 days. Ｗe can observe Cells
maintained a round colony condition and the circumferential department cells. Cytokeratin8 and
Nestin which were neural progenitor cell marker were also observed under fleuroscence
microscope.
Maintenance and characterization method of neural progenitor cells were established but, not
neural cells. For the further study, we will progress differentiation study to neural cell in second
project, "Development and application of embryonic stem cell models for the evaluation of
chemical toxicity." We expect each established stem cell lines and differentiation cell lines can be
important materials for wide range of biotechnology research, toxicity screening test and disease
model development.
Keyword
Embryonic stem cell, EC cell, EG cell, embryoid body, differentiated cell
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