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Isothermal?amplification?detection?
LAMP?(Loop?mediated?isothermal?????????????????
platforms
amplification)?
LAMP?(Loop?mediated?isothermal?????????????????
LAMP?(Loop?mediated?isothermal?????????????????
amplification)?
amplification)?
Bst DNA polymerase with strand displacement
Theamplificationefficiencyisextremelyhigh
activityat65
Reducedtotalcost-notrequirespecialreagentsor
The whole amplification to 10
9
? 10
10
copies is
sophisticatedequipments
finishedwithin15to60minat65 ,isothermall
Amplified products have a structure consisting of
y
alternatelyinvertedrepeatsofthetarget
Amplification and detection of gene can be
sequenceonthesamestrand
completedinasinglestep
AmplificationcanbedonewithRNAtemplatesfoll
No need for a step to denature double
owingthesameprocedureaswithDNAtemplates
strandedintoasinglestrandedform
, simply through the addition of reverse
transcriptase(RT-LAMP)
LAMP?(Loop?mediated?isothermal?????????????????
platforms
amplification)?
LAMP?(Loop?mediated?isothermal?????????????????
LAMP?(Loop?mediated?isothermal?????????????????
amplification)?
amplification)?
Bst DNA polymerase with strand displacement
Theamplificationefficiencyisextremelyhigh
activityat65
Reducedtotalcost-notrequirespecialreagentsor
The whole amplification to 10
9
? 10
10
copies is
sophisticatedequipments
finishedwithin15to60minat65 ,isothermall
Amplified products have a structure consisting of
y
alternatelyinvertedrepeatsofthetarget
Amplification and detection of gene can be
sequenceonthesamestrand
completedinasinglestep
AmplificationcanbedonewithRNAtemplatesfoll
No need for a step to denature double
owingthesameprocedureaswithDNAtemplates
strandedintoasinglestrandedform
, simply through the addition of reverse
transcriptase(RT-LAMP)
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