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과 제 명 야외감염과 감별가능한 뉴캣슬병 백신(마커백신)주 개발
연
Generation and Biological Characterization of a Neutralization- Resistant
구
발표제목
Mutant of Newcastle Disease Virus
성
과
Mi-Ja Park, Soo-Jeong Kye, Ji-Ye Kim, Saeromi Kim, Hee-Jung Seul, Choi-Kyu
발 표 자
Park, Kang-Seuk Choi
활
용
수록잡지 Journal of Bacteriology and Virology 42(4) : 330-338
발표시기
2012년 12월
집
Abstract :
A neutralization-resistant mutant of Newcastle disease virus(NDV) Kr005 strain belonging
to class II genotype VII was generated using a neutralizing monoclonal antibody and
its biological effects were assessed. The mutant showed single amino acid
substitution(E to K) at position 347 of the hemagglutinin-neuraminidase(HN)
protein(E347K mutant). The E347K mutant exhibited marked rounding of the cells and
few syncytia in infected chicken embryofibroblast(CEF) cells. The hemadsorption and
neuraminidase activities of the E347K mutant of the wild-type virus were 118% and
166%, respectively. The mutant produced a rapid elution pattern whereas the wild type
had a slow elution pattern. Growth kinetics studies showed that the E347K mutant
produced an 80-times higher yield of extracellular virus in CEF cells compared with
the wild-type virus. The time-course virus titer showed a marked increase in
mutant-infected cells from 6 h to 12 h post infection(pi), which was consistent with
the titer pattern time-course for NA activity. The E347K mutant virus showed slight
decrease in virulence compared to the wild-type virus but there was no change in
pathotype when measured by in vivo pathogenicity testing. These results suggest that
an E347K mutation in HN protein might be associated with increased NA activity and
subsequent enhancement of virus release from infected cells without change in viral
pathotype.
882
연
Generation and Biological Characterization of a Neutralization- Resistant
구
발표제목
Mutant of Newcastle Disease Virus
성
과
Mi-Ja Park, Soo-Jeong Kye, Ji-Ye Kim, Saeromi Kim, Hee-Jung Seul, Choi-Kyu
발 표 자
Park, Kang-Seuk Choi
활
용
수록잡지 Journal of Bacteriology and Virology 42(4) : 330-338
발표시기
2012년 12월
집
Abstract :
A neutralization-resistant mutant of Newcastle disease virus(NDV) Kr005 strain belonging
to class II genotype VII was generated using a neutralizing monoclonal antibody and
its biological effects were assessed. The mutant showed single amino acid
substitution(E to K) at position 347 of the hemagglutinin-neuraminidase(HN)
protein(E347K mutant). The E347K mutant exhibited marked rounding of the cells and
few syncytia in infected chicken embryofibroblast(CEF) cells. The hemadsorption and
neuraminidase activities of the E347K mutant of the wild-type virus were 118% and
166%, respectively. The mutant produced a rapid elution pattern whereas the wild type
had a slow elution pattern. Growth kinetics studies showed that the E347K mutant
produced an 80-times higher yield of extracellular virus in CEF cells compared with
the wild-type virus. The time-course virus titer showed a marked increase in
mutant-infected cells from 6 h to 12 h post infection(pi), which was consistent with
the titer pattern time-course for NA activity. The E347K mutant virus showed slight
decrease in virulence compared to the wild-type virus but there was no change in
pathotype when measured by in vivo pathogenicity testing. These results suggest that
an E347K mutation in HN protein might be associated with increased NA activity and
subsequent enhancement of virus release from infected cells without change in viral
pathotype.
882
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