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과 제 명 야외감염과 감별가능한 뉴캣슬병 백신(마커백신)주 개발
논
문
발표제목 Vero 세포적응 뉴캣슬병 바이러스의 생물학적 특성
게
발 표 자 최강석, 박미자, 계수정, 김지예, 권준헌
재
?
수록잡지 한국가금학회지 39(2) : 113-120
발표시기
2012년 6월
비
S
Abstract :
C
I
Newcastle disease virus(NDV) Kr005/V strain was generated through 55 serial passages
?
of NDV Kr005 strain in Vero cells. The Kr005/V virus yielded high infective titers of
107.8 TCID50/㎖ in Vero cells and the infected cells showed cytopathic effects such as
marked cell rounding, though less frequent syncytia. The Kr005/V virus was heat-stable
and classified into the lentogenic type with a Mean Death Time(MDT) of 120h or
greater while the Kr005 strain was heat-labile and velogenic(MDT of 49.6 h). Only the
single amino acid substitution(T to S) was observed at position 433 of the HN protein
of the Kr005/V strain, whereas no amino acid change was found in the F protein. The
Kr005/V input virus correlated well(correlation coefficient r2=0.97) with the Kr005 virus
when ten field sera were tested by virus neutralization test. The biological properties
and usefulness of Vero cell-adapted Kr005/V virus were discussed.
885
논
문
발표제목 Vero 세포적응 뉴캣슬병 바이러스의 생물학적 특성
게
발 표 자 최강석, 박미자, 계수정, 김지예, 권준헌
재
?
수록잡지 한국가금학회지 39(2) : 113-120
발표시기
2012년 6월
비
S
Abstract :
C
I
Newcastle disease virus(NDV) Kr005/V strain was generated through 55 serial passages
?
of NDV Kr005 strain in Vero cells. The Kr005/V virus yielded high infective titers of
107.8 TCID50/㎖ in Vero cells and the infected cells showed cytopathic effects such as
marked cell rounding, though less frequent syncytia. The Kr005/V virus was heat-stable
and classified into the lentogenic type with a Mean Death Time(MDT) of 120h or
greater while the Kr005 strain was heat-labile and velogenic(MDT of 49.6 h). Only the
single amino acid substitution(T to S) was observed at position 433 of the HN protein
of the Kr005/V strain, whereas no amino acid change was found in the F protein. The
Kr005/V input virus correlated well(correlation coefficient r2=0.97) with the Kr005 virus
when ten field sera were tested by virus neutralization test. The biological properties
and usefulness of Vero cell-adapted Kr005/V virus were discussed.
885
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