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연
One-step Multiplex RT-PCR Assay for the Detection of West Nile and
구
발표제목
Japanese Encephalitis Viruses
성
과
발 표 자 조윤상, 예정용, 이지혜, 박지용, 조인수
Vaccines & Diagnostics for Transboundary
활
발 표 처
발표시기
2012년 9월
Animal Diseases
용
집
Abstract :
Objectives
West Nile virus (WNV), the cause of West Nile fever and West Nile encephalomyelitis,
is a flavivirus antigenically similar to Japanese encephalitis virus (JEV), St. Louis
encephalitis virus, and Murray Valley encephalitis virus. Species within the Flavivirus
genus can cause public health problems around the world. WNV and JEV are
arthropod-borne viruses that can emerge or re-emerge in many regions due to climate
change and increased travel. WNV and JEV are associated with both human and
equine encephalitis worldwide. The aim of this study was to develop a highly
sensitive and specific one-step duplex reverse transcriptase-polymerase chain reaction
for the simultaneous and differential detection of WNV and JEV.
Methods
Bioinformatics analysis of published sequences of WNV and JEV revealed conserved
regions not targeted by previously reported primers. A total of thirteen primers were
designed based on these regions to detect all of the WNV and JEV lineages, and to
discriminate between the two viruses by the generation of 482 and 241 base pair
cDNA products, respectively.
Results and Conclusions
The results indicate that single-tube duplex reverse transcriptase-polymerase chain
reaction using these primers is a useful technique for the detection and differentiation
of WNV and JEV in plasma or brain tissue. The novel duplex reverse
transcriptase-polymerase chain reaction described in this study enables the early
diagnosis of these two encephalitic flaviviruses. In addition, this technique may be
useful as part of a testing regimen for human patients, horses, and other susceptible
animal species as it is rapid (less than 3.5 h from RNA extraction), sensitive and
specific, and may enable differential diagnosis of clinical samples. Footnote: The data
included in this presentation were published in J Clin Microbiol. 2010
Nov;48(11):4010-4.
1300
연
One-step Multiplex RT-PCR Assay for the Detection of West Nile and
구
발표제목
Japanese Encephalitis Viruses
성
과
발 표 자 조윤상, 예정용, 이지혜, 박지용, 조인수
Vaccines & Diagnostics for Transboundary
활
발 표 처
발표시기
2012년 9월
Animal Diseases
용
집
Abstract :
Objectives
West Nile virus (WNV), the cause of West Nile fever and West Nile encephalomyelitis,
is a flavivirus antigenically similar to Japanese encephalitis virus (JEV), St. Louis
encephalitis virus, and Murray Valley encephalitis virus. Species within the Flavivirus
genus can cause public health problems around the world. WNV and JEV are
arthropod-borne viruses that can emerge or re-emerge in many regions due to climate
change and increased travel. WNV and JEV are associated with both human and
equine encephalitis worldwide. The aim of this study was to develop a highly
sensitive and specific one-step duplex reverse transcriptase-polymerase chain reaction
for the simultaneous and differential detection of WNV and JEV.
Methods
Bioinformatics analysis of published sequences of WNV and JEV revealed conserved
regions not targeted by previously reported primers. A total of thirteen primers were
designed based on these regions to detect all of the WNV and JEV lineages, and to
discriminate between the two viruses by the generation of 482 and 241 base pair
cDNA products, respectively.
Results and Conclusions
The results indicate that single-tube duplex reverse transcriptase-polymerase chain
reaction using these primers is a useful technique for the detection and differentiation
of WNV and JEV in plasma or brain tissue. The novel duplex reverse
transcriptase-polymerase chain reaction described in this study enables the early
diagnosis of these two encephalitic flaviviruses. In addition, this technique may be
useful as part of a testing regimen for human patients, horses, and other susceptible
animal species as it is rapid (less than 3.5 h from RNA extraction), sensitive and
specific, and may enable differential diagnosis of clinical samples. Footnote: The data
included in this presentation were published in J Clin Microbiol. 2010
Nov;48(11):4010-4.
1300
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