총 808페이지
358페이지 본문시작
1-4-2-01
<
부 기
>
부기
1.
세포증식용 배지
(Hank's balanced salt solution)
Sodium chloride .......................................................................................................... 8.0 g
Potassium chloride ..................................................................................................... 0.4 g
Calcium chloride ....................................................................................................... 0.14 g
Magnesium sulfate heptahydrate .......................................................................... 0.2 g
Potassium dihydrogen phosphate ........................................................................ 0.06 g
Sodium hydrogen phosphate ............................................................................ 0.0475 g
Glucose .......................................................................................................................... 1.0 g
Phenol red .................................................................................................................. 0.02 g
Lactalbumin hydrolysate .......................................................................................... 5.0 g
Streptomycin sulfate ......................................................................................... 0.2mg/ml
D.W ............................................................................................................................ 1,000ml
Calf serum ............................................................................................................... 5
10%
부기
2.
세포유지용 배지
상기 세포증식용 배지에
calf serum
2%
되게 감량 첨가한다
부기
3.
보호제
LPGG(lactose, phosphate, glutamate, gelatin mixture)
Lactose ........................................................................................................................ 74.62g
........................................................................................................................ 0.517g
2
........................................................................................................................ 0.854g
2
Sodium L-glutamate .................................................................................................... 10g
Gelatin ................................................................................................................................ 5g
D.W ............................................................................................................................ 1,000ml
<
부 기
>
부기
1.
세포증식용 배지
(Hank's balanced salt solution)
Sodium chloride .......................................................................................................... 8.0 g
Potassium chloride ..................................................................................................... 0.4 g
Calcium chloride ....................................................................................................... 0.14 g
Magnesium sulfate heptahydrate .......................................................................... 0.2 g
Potassium dihydrogen phosphate ........................................................................ 0.06 g
Sodium hydrogen phosphate ............................................................................ 0.0475 g
Glucose .......................................................................................................................... 1.0 g
Phenol red .................................................................................................................. 0.02 g
Lactalbumin hydrolysate .......................................................................................... 5.0 g
Streptomycin sulfate ......................................................................................... 0.2mg/ml
D.W ............................................................................................................................ 1,000ml
Calf serum ............................................................................................................... 5
10%
부기
2.
세포유지용 배지
상기 세포증식용 배지에
calf serum
2%
되게 감량 첨가한다
부기
3.
보호제
LPGG(lactose, phosphate, glutamate, gelatin mixture)
Lactose ........................................................................................................................ 74.62g
........................................................................................................................ 0.517g
2
........................................................................................................................ 0.854g
2
Sodium L-glutamate .................................................................................................... 10g
Gelatin ................................................................................................................................ 5g
D.W ............................................................................................................................ 1,000ml
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