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In this study, the research team aimed to explore the role of serine protease
inhibitors in defending tea plants against herbivorous pests. Initially, researchers
conducted a thorough screening of tea plant accessions to assess their resistance to
the tea geometrid, a major pest. This involved identifying and analyzing the
expression patterns of four SERPIN genes from the tea genome, focusing on their
structure and homology to known SERPINs in other plant species. Among these,
CsSERPIN1 is noteworthy for its negative correlation with herbivore growth, indicating
a potential role in pest resistance.
Further investigation into CsSERPIN1 revealed that it is a constitutively expressed
gene with stable expression levels across various biotic and abiotic stress conditions.
The functionality of CsSERPIN1 as a protease inhibitor was then tested through in
vitro experiments, where it demonstrated significant inhibitory effects on the digestive
proteases trypsin and chymotrypsin. These findings were corroborated by in vivo
assays, showing that larvae fed on diets containing CsSERPIN1 had reduced growth
and protease activity. The defensive capabilities of CsSERPIN1 were also investigated
by transiently overexpressing it in tea plants and heterologously expressing it in
Arabidopsis. Both experiments resulted in decreased herbivory by the tea geometrid
and fall armyworm, respectively.
In conclusion, this research highlights the role of CsSERPIN1 as a novel,
constitutively expressed serine protease inhibitor that effectively reduces herbivore
growth and development without adversely affecting the host plant's growth. By
demonstrating CsSERPIN1's broad-spectrum activity against various herbivores and its
stable expression unaffected by environmental factors, the study suggests CsSERPIN1
is a promising candidate for developing pest-resistant tea plant varieties.
The implications of this research extend beyond tea agriculture, offering insights into
the potential of constitutive protease inhibitors as a durable and eco-friendly solution
for crop protection. More information: Meng Ye et al, A constitutive serine protease
inhibitor suppresses herbivore performance in tea (Camellia sinensis), Horticulture
Research (2023). DOI: 10.1093/hr/uhad178
- 367 -
inhibitors in defending tea plants against herbivorous pests. Initially, researchers
conducted a thorough screening of tea plant accessions to assess their resistance to
the tea geometrid, a major pest. This involved identifying and analyzing the
expression patterns of four SERPIN genes from the tea genome, focusing on their
structure and homology to known SERPINs in other plant species. Among these,
CsSERPIN1 is noteworthy for its negative correlation with herbivore growth, indicating
a potential role in pest resistance.
Further investigation into CsSERPIN1 revealed that it is a constitutively expressed
gene with stable expression levels across various biotic and abiotic stress conditions.
The functionality of CsSERPIN1 as a protease inhibitor was then tested through in
vitro experiments, where it demonstrated significant inhibitory effects on the digestive
proteases trypsin and chymotrypsin. These findings were corroborated by in vivo
assays, showing that larvae fed on diets containing CsSERPIN1 had reduced growth
and protease activity. The defensive capabilities of CsSERPIN1 were also investigated
by transiently overexpressing it in tea plants and heterologously expressing it in
Arabidopsis. Both experiments resulted in decreased herbivory by the tea geometrid
and fall armyworm, respectively.
In conclusion, this research highlights the role of CsSERPIN1 as a novel,
constitutively expressed serine protease inhibitor that effectively reduces herbivore
growth and development without adversely affecting the host plant's growth. By
demonstrating CsSERPIN1's broad-spectrum activity against various herbivores and its
stable expression unaffected by environmental factors, the study suggests CsSERPIN1
is a promising candidate for developing pest-resistant tea plant varieties.
The implications of this research extend beyond tea agriculture, offering insights into
the potential of constitutive protease inhibitors as a durable and eco-friendly solution
for crop protection. More information: Meng Ye et al, A constitutive serine protease
inhibitor suppresses herbivore performance in tea (Camellia sinensis), Horticulture
Research (2023). DOI: 10.1093/hr/uhad178
- 367 -
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