총 808페이지

1-4-4-09
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부 기
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부기
1.
세포증식용 배지
Eagle's MEM
또는
alpha MEM
Tryptose phosphate broth ........................................................................................... 5%
Fetal bovine serum ..................................................................................................... 10%
7.5% sodium bicarbonate solution ............................................................................ 2%
L-glutamine ..................................................................................................................... 3%
Fungizone ........................................................................................................... 0.025
Penicillin G-Na ................................................................................................. 200unit/ml
Streptomycin ......................................................................................................... 100
부기
2.
세포유지용 배지
Eagle's MEM
또는
alpha MEM
Tryptose phosphate broth ........................................................................................... 5%
Fetal bovine serum ....................................................................................................... 5%
7.5% sodium bicarbonate solution ............................................................................ 2%
L-glutamine ..................................................................................................................... 3%
Fungizone ........................................................................................................... 0.025
Penicillin G-Na ................................................................................................. 200unit/ml
Streptomycin ......................................................................................................... 100
부기3. Ellinghausen's semisolid oleic albumin medium
가 저장용액
1) 25
배 인산염완충식염수
.......................................................................................................................... 16.6g
2
.......................................................................................................................... 2.172g
2
4
D.W .............................................................................................................................. 1,000ml
2) 20
배 식염액
NaCl ................................................................................................................................. 38.5g
Cl ............................................................................................................................... 5.35g
NaCl
6H
..................................................................................................................
3.81g
2
D.W .............................................................................................................................. 1,000ml
3) Cu
용액
CuSO
.............................................................................................................................. 30mg
D.W ................................................................................................................................. 100ml
4) Zn
용액
ZnSO
7H
O ................................................................................................................ 80mg
D.W ................................................................................................................................. 200ml
5) Fe
용액
FeSO
7H
O ................................................................................................................. 80mg
D.W ................................................................................................................................. 200ml

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