총 808페이지
431페이지 본문시작
1-4-4-09
부기5. Leptospira medium
가 기본배양액
............................................................................................................................. 54g
2
............................................................................................................................ 16.2g
2
4
NaCl .................................................................................................................................... 54g
Cl ............................................................................................................................... 13.5g
Vitamin B
..................................................................................................................... 0.27g
Sodium pyruvate ............................................................................................................ 5.4g
Glycerol ......................................................................................................................... 15.4ml
Tween80 .......................................................................................................................... 75ml
D.W ............................................................................................................................ 60,000ml
여과멸균
나. BSA solution
Bovine serum albumin ................................................................................................ 700g
CaCl
................................................................................................................................ 1.05g
MgCl
............................................................................................................................... 1.05g
ZnSO
............................................................................................................................... 0.28g
CuSO
............................................................................................................................ 0.021g
FeSO
................................................................................................................................. 3.5g
Vitamin B
.................................................................................................................. 0.014g
12
D.W .............................................................................................................................. 7,000ml
121
30
Autoclave. pH 7.2
7.4
부기
6.
보호제
LPGG(lactose, phosphate, glutamate, gelatin mixture)
Lactose ........................................................................................................................ 74.62g
........................................................................................................................ 0.517g
2
........................................................................................................................ 0.854g
2
Sodium L-glutamate .................................................................................................... 10g
Gelatin ................................................................................................................................ 5g
D.W ............................................................................................................................ 1,000ml
부기7. PBS(phosphate buffered saline)
NaCl ................................................................................................................................. 8.0g
KCl ................................................................................................................................... 0.4g
.......................................................................................................................... 0.06g
2
.................................................................................................................... 0.0475g
2
4
D.W ............................................................................................................................ 1,000ml
부기5. Leptospira medium
가 기본배양액
............................................................................................................................. 54g
2
............................................................................................................................ 16.2g
2
4
NaCl .................................................................................................................................... 54g
Cl ............................................................................................................................... 13.5g
Vitamin B
..................................................................................................................... 0.27g
Sodium pyruvate ............................................................................................................ 5.4g
Glycerol ......................................................................................................................... 15.4ml
Tween80 .......................................................................................................................... 75ml
D.W ............................................................................................................................ 60,000ml
여과멸균
나. BSA solution
Bovine serum albumin ................................................................................................ 700g
CaCl
................................................................................................................................ 1.05g
MgCl
............................................................................................................................... 1.05g
ZnSO
............................................................................................................................... 0.28g
CuSO
............................................................................................................................ 0.021g
FeSO
................................................................................................................................. 3.5g
Vitamin B
.................................................................................................................. 0.014g
12
D.W .............................................................................................................................. 7,000ml
121
30
Autoclave. pH 7.2
7.4
부기
6.
보호제
LPGG(lactose, phosphate, glutamate, gelatin mixture)
Lactose ........................................................................................................................ 74.62g
........................................................................................................................ 0.517g
2
........................................................................................................................ 0.854g
2
Sodium L-glutamate .................................................................................................... 10g
Gelatin ................................................................................................................................ 5g
D.W ............................................................................................................................ 1,000ml
부기7. PBS(phosphate buffered saline)
NaCl ................................................................................................................................. 8.0g
KCl ................................................................................................................................... 0.4g
.......................................................................................................................... 0.06g
2
.................................................................................................................... 0.0475g
2
4
D.W ............................................................................................................................ 1,000ml
431페이지 본문끝
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