총 508페이지

RT&Nested-PCR 검사법
28. RpRSV
RpRS-N37
CAAAGAGTGCGCTGTTCCATA
*set 1
721 bp
RpRS-C15
GGTGCATATTCTTTCAAGGGAGGAT
RpRS-N39
GCTGAGGCTGGGTGGAATAATG
*set 1
322 bp
nested
RpRS-C18
GCGAAGAAAAGCTCAACATAG
RpRS-N35
GGCCAATGATCCCAATAAAGTT
set 2
750bp
RpRS-C15
GGTGCATATTCTTTCAAGGGAGGAT
RpRS-N37
CAAAGAGTGCGCTGTTCCATA
set 2
353 bp
nested
RpRS-C20
GGGCATATCAGAACACGCACCATC
RpRS-N30
TTCCCGCTTGTATGATGACTGACCA
set 3
645 bp
RpRS-C19
AAAGGTAAACTCAGCAGCGGAAGAT
RpRS-N35
GGCCAATGATCCCAATAAAGTT
set 3
280 bp
nested
RpRS-C32
CGCATTATTCCACCCAGCCTCAGC
[TTCCTGCGTGCATGATGACTGACCA]TCACCTACAACCAACGGGTGATAGGCAGGCCGACAAGGAAGAGCG
GCAGGATTATGCGGACAGCCAGGACTCTATTCAGAGCATGGGTGATTTCTGGAAGGAATTCTATTCAAAGGA
TTCTGGAAAGAAAATCCCTGACTCCCACAAGTCAAGGCT[]GGTTTTA[
GAAGAGTGCGCTTTTCCATA>]AGCAGCCCCTGGCGCATTCATTAGCTCAAACTTGGGCTAACTTTAGGGGTA
CCCAGGATAAGGCGGACCTGGTCAAAGTGACCATGGACATGAATATTGAGAAATATACTGTCCGTCTTCCCG
ATGCAGTTCGTACTACGGCAGGACCACTATACATTGAGTGGATTAATCTGCCACGAATGTCTGAAAATTCTGC
ACGTAAACTACA>GATATTTGTGGGGTGGATCTTGCCATGAAGTCGCAT
GTTGCAGTTGGCACGCCAGTCCGTGTTATCATCTCTTTGGTAAC{CTC
GAG}GGCAACGATGTGCGCTTTTGAGGTGAACTTGGCAACACAGAACAACCGGTCACTAA[ATCTTCCGCTGC
TTAGTTTACCCTT]CTCACAACTGCTCGCCGATTTGCATGATTTCCAACATCGTGTCAAGATCGCTTGCCAATT
TCGGGACCCGGAAGGATTCAATGTGGGTACTCTCGAATTTTCTGAGCTGAA
GCAAACCGCTTTCGAGCGGAAATCTTTACTCAGAGATTCCTGGTCTGAAATAGAAAAGCGTGCTTGCCATGG
TGGAGGACGTTGTATCGCCTCCCAGGGTATTGTGCAGACCTGGGAGAAGGAGATTA[ATCCTCCTTTAAAAG
AATATGCACC]
[_], RT-PCR primer binding site; ,Nested-PCR primer binding site; { }, mutation site;
*표시된 primer set를 사용하면 양성대조구로부터의 오염여부 확인 가능
438 ?

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