총 508페이지

RT&Nested-PCR 검사법
31. SqMV
!돌연변이 양성대조구 염기서열 변경
SqM-N20
CCGAAAAAGCTGTGGTCAATCA
*set 1
680 bp
SqM-C40
TTGGACAATGGCCCCCTTTTC
SqM-N30
TAAAATGTATGAGCGAAGTAAGAG
*set 1
568 bp
nested
SqM-C45
AAACTAACAGCGCAACCTGC
SqM-N10
ATTTGGTGGGAGATTATTCA
*set 2
852 bp
SqM-C40
TTGGACAATGGCCCCCTTTTC
SqM-N20
CCGAAAAAGCTGTGGTCAATCA
*set 2
680 bp
nested
SqM-C40
TTGGACAATGGCCCCCTTTTC
[ATTTGGTGGGAGATTATTCA]AATACTTCACCATTTATTCCAGTACAAGGAACCAGCATTATTTATTAATAGCTGT
CAGGATCTTGCTGCTTTTTTAGAGAGGAAACATTCCATGGAAGTGATTCAGAAGGAAGGTTTGGCTGCTTCAGC
ACTCAAGGATAAGGAGCGACTGG[]GCCCCTGAGTAACTTGATTCCTCATT
CAAATCTTCTATCTGGTCTTAAGCGTGGTTTGATAAAGCAAAAAGAGGT
AGCTTTTGATAAGCTCATGGGGGGTTCGACAATAGACTTCCAGCATATTCCAACCGGAACTCTCACACCTGGTG
AGAATAAGGTGCTAGACATACCAATTGTCCCGCAACATTTATTGACCAGTACGAATATAACGGATTACCATCAG
G{ACGCGT}CCAATAATAAGAGTGCTAATGGTGCTACTGCACTTCATGTTGGAGCTATAGAAGTCATCTTAGATT
GCTTTACCTCTCCTGATAGTAACATTTGTGGTGGTATGTTGCTGGTTGATACAGCACATTTAAACCCGGATAATG
CAATAAGAAGTGTTTTTGTCGCGCCATTTATTGGTGGTCGCCCTATTCGAGTTTTGTTGTTTCCAGACACTTTGGT
GGAAATTGCCCCAAACATGAATTCTCGATTTAAATTGCTATGTACTACTAGCAATGGCGACGTTGCACCAGATTT
TAATTTAGCGATGGTCAAAGTCAATGTTGACCAAGACATACACTCCTACAGCC
TACCTTGAGCAAGAGCTAATTAAA[]
32. TBRV
!돌연변이 양성대조구 염기서열 변경
TBR-N40
ACTGTGTTAAATCGCCCGCA
*set 1
510 bp
TBR-C30
TGCCAGCCTCAACGAAATC
TBRN-N40
TGGAARGAGGCAAAGTCTGT
*set 1
313 bp
nested
TBRN-C30
CAACRGCAGTGATTCCAAACAT
TBR-N20
GCAGTCTGTTCACCTGCG
*set 2
662 bp
TBR-C70
AGATGCACGGGGAGATAACT
TBRN-N20
AGTTTGTCGAGGGTTCTGA
*set 2
307 bp
nested
TBRN-C70
TTCCATCCCGCTTCAGCAAGT
[GCAGTCTGTTCACCTGCG]AAACTCCTACCTGAAATTGGGGAGAGCGACTTTATTGAGTTGGAAGAAGAGGA
AACTATTTCTATTCCTCCTCTCTTTAAGGACAATGGTCTGTCTGAACTATATATGAATCGCATATTAAAGCAGG
CTGTTGTGACACCAACTCTATGGCACACCCTGACCAGGATGAAACTGAGGATCAACTTCATCATCTCGAGAA
CAAACTGGGTGGAGAAATTGTCTCAACTCCTTCCTTCATCAAAATGCTGAAGGATAAGCGCAAAGAAGTGCG
CGGGAAGGGGGTCGCCTAGTTCGCTCAGCAGATCTTGAACTGAGCAAAAAA
GATATCTTCCTTGCCCATACTTTGATGGATAAATTCCATGGTATGAGCATTGTTAAGAAATTTGGGAAGAGCG
ACCCAAAATTGACTAAAGTTTGTGTTGATTTGACCAACCAGGAGGAGGTGATACGATTCC{TCGACG}CTGCA
AAGGAATTGCAAACCACTTCTGAAGGTGTTTTATCTGCACAAACTTTT[ACTGTGTTAAATCGCCCGCA]ATTTA
AAGAATTGAATAATTGTCTCAATTTACACATTCG
G[AGTTATCTCCCCGTGCATCT]GCCTGTCTACGCTTTTTGCGTTATCATGTGGGGGCATTCTTCGAAT{TCGAC
G}GCTGAACAGGCCAGTCTTAGTGGAGCCTATGTATATCTCGGAGATCAAGACGCCTCTGTTTTACAGTTGCC
CCTACTCTGTGGATATATTGGTGACGCTCTGGAAGACATGGAAGCATACAAGCGATCACTCGTCTTGTCTAC
ATGCTTCTTTGGCACTTCAGGACTTTCCGCTGGCCAGAGTAGTTTACTGA
GTACCTCCCTACCTCGTATGGAGGGATTACACACGAACGTGATTCCTGGAACCAAATGTTGCGGAACCACCA
AGGTGTTGACAAACAGAGATTTATCTCTGGATTCAACGTGGTA[GATTTCGTTGAGGCTGGCA]
[_], RT-PCR primer binding site; ,Nested-PCR primer binding site; { }, mutation site;
*표시된 primer set를 사용하면 양성대조구로부터의 오염여부 확인 가능
440 ?

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