총 508페이지

RT&Nested-PCR 검사법
35. TRSV
!돌연변이 양성대조구 염기서열 변경
TRSV­N10
GTTGTTCCCGATCCCACTTGTTG
*set 1
691 bp
TRSV­C80
TAACTGCCCGGGAATATGAAATGG
TRSV­N12
CCCAAAGATGCGAAGAAAGGAA
*set 1
414 bp
nested
TRSV­C90
GCCAATCAGCAGCCATCGTC
TRSV­N30
TCCGAGATGTTTGAACTGCCTACC
*set 2
580 bp
TRS­C30
TGGCCATCTCCGTGCATTATCTGA
TRSV­N40
CATTGATCGTTTTGAGTTAGTTCC
*set 2
234 bp
nested
TRSV­C70
CTTCCGCTTATAGTGCCAGACCA
[GTTGTTCCCGATCCCACTTGTTG]TGGGACATTGTCCTTTAAGGTTAGC
ATCTTGGAACTTTTGACATTCGGCAAGCCATTATGGAATATGGTGGTTTGCATTCCCAAGAATGGTGTGCAAA
GGGCATTGTTAATCCCACTTTTACAGTGAGGATGCATGCCCCACGCAACGCCTTTGCAGGTTTGTCTATAGCG
TGCACCTTTGATGAT{CTCGAG}TACAAACGCATAGACTTACCAGCGCTTGGGAATGAATGTCCTCCC[TCCGA
GATGTTTGAACTGCCTACC]AAGGTTTTCATGCTTAAAGATGCAGATGTGCATGAATGGCAGTTCAACTATGG
GGAACTTACAGGACATGGGTTGTGCAATTGGGCAAATGTAGTTACCCAGCCCACATTGTACTTTTTTGTTGCG
TCCACAAATCAAGTAGTGTATTGTTACTATGCATGTGGACATGGGGCCCGT
AACTATGACGTGGCCTATTCAATTGGGTGACACTTTCGCCATTGATAG
ATATTATGAGGCGAAAGAAATTAAACTTGACGGGTCAACCTCCATGT{CTCGAG}TGTCCATATCTTATAATTTT
GGAGGTCCCGTCAAGCATTCTAAGAAACATG[CCATTTCATATTCCCGGGCAGTTA]TGTCTAGGAATCTTGG
GTGTCAAGAGTGTTTCTTCTTTATTTTGTACCGCTTCTTTTGTTATTTTTC
CATGGGAACATGAAGCACCTCCAACCTTACGTCAGGTGTTATGGGGCCCACA[TCAGATAATGCACGGAGAT
GGCCA]
36. TSV
set 2는 양성대조구가 포함하지 않음
TS-N20
GTCCAGACCATCCATCCAACG
*set 1
842 bp
TS-C10
GCGCGGCAGCTATGCATACT
TSN-N20
CGACGCCAGCGGCGTAATGCC
*set 1
538 bp
nested
TSN-C10
AAATCTTCTGGTGGCATCAAGG
TS-N10
CTATTGAGAAGTCGTGCCT
set 2
812 bp
TS-C30
CCATCAGAGACGGCGACAAA
TS-N20
GTCCAGACCATCCATCCAACG
set 2
424 bp
nested
TS-C30
CCATCAGAGACGGCGACAAA
[GTCCAGACCATCCATCCAACG]CCATGTCTTCGCGTGCTAACAACCGCTCAAATAACAGCAGATGCCCAACT
TGCATTGATGAGTTGGACGCTATGGCCAGGAATTGTCCCGCCCATAATACCGTGAACACTGTTTCA
CCAGCGGCGTAATGCC>GCTAGAGCTGCCGCGTATAGAAACGCGAATGCTAGAGTACCGCTACCGCTTCCT
GTGGTATCGGTTTCCCGCCCTCAAGCGAAGGCGTCGTTGAGGTTACCCAACAATCAAGTTTGGGTAACTCGC
AAAGCGAGTGAATGGTCTGCAAAGACAGTTGATACCAACGATGCTATCCCCTTCAAAACCATAGTCGAGGG
GATTCCCGAAATCGGTGCTGAGACGAAGTTTTTCCGTCTCTTGATCGGTTTTGTCGCCGTCTCAGATGGGACG
TTTGGGATGGTTGATGGAGTAACAGGCGATGTCATTCCTGACCCACCAGTCGTAGGACGGTTGGGTTTCAAG
AAGAATACATACCGCAGCCGAGACTTTGATCTCGGTGGTAAACTCCTCAATCAACTAGACGACAGAGCTGTC
{CTCGAG}GTCTGGTGCCTCGACGAACGTCGTCGAGAAGCCAAGAGGGTTCAGCTGGCAGGATATTGGATCG
CAATTTCTAAACCAGCTCTCTGGTGAATCAAGACTGACTAGATGGTCAC
CTCGGTGAGACCGAGATGCCGCACATAGATGGTAATTCCTGTGTGCGATGTGGATTCACACCTCGACGTATT
GTAGATCAATACATTTGGAAGTGTGACATCTGTTCCTATGAAC[AGTATGCATAGCTGCCGCGC]
[_], RT-PCR primer binding site; ,Nested-PCR primer binding site; { }, mutation site;
*표시된 primer set를 사용하면 양성대조구로부터의 오염여부 확인 가능
442 ?

현재 포커스의 아래내용들은 동일한 컨텐츠를 가지고 페이지넘김 효과및 시각적 효과를 제공하는 페이지이므로 스크린리더 사용자는 여기까지만 낭독하시고 위의 페이지이동 링크를 사용하여 다음페이지로 이동하시기 바랍니다.