총 508페이지

37. WSMV
!돌연변이 양성대조구 염기서열 변경
WSMV­N20
TGGCGATGAAGATGTCAG
*set 1
834 bp
WSMV­C80
CCATTTCTGTGAAGGCTTT
WSMV­N30
GCGTGGTAACGAATACACATACTA
*set 1
299 bp
nested
WSMV­C80
CCATTTCTGTGAAGGCTTT
WSMV­N30
GCGTGGTAACGAATACACATACTA
*set 2
672 bp
WSMV­C70
TTCTCCTCTCTGTTCCTCATA
WSMV­N30
GCGTGGTAACGAATACACATACTA
*set 2
299 bp
nested
WSMV­C80
CCATTTCTGTGAAGGCTTT
[TGGCGATGAAGATGTCAG]CAAATCCTGAAGATGTGGGAACCATATTGTATATGGTTGAGCAAGCGCTAATA
AGTGAAAAGACGAAACTGGAGAATTTAACCCAGGCATTCCAGCAGCAACAATCAACATATTGCAGCGTGCTC
ATCCCTAATTTTAATGTTGCTGGTCGGCTAACACAGGCAATGGATCGCATAAGAAAGAATGTTTCTGTGCTGC
AACACCAGAAAACAGCTCTCGAAAAGGCGGCAGTGACTTACGACTACACGAAGTTAGTTGAGCTCCTTGATG
AAAATCCAAGCATAGCTTCCCATGTGTCATACCAGGCTGGGCCAGCAAAATTCATTGATGAATTCATATTGGA
GAAGCGCGATTATGGGTGGCTACCATATCTTGCAGTAGGAACTGCGTGTGCAATTGCTGGCACAACACTTGT
AATGATGTACTACCGTCGCATGAAGCGTAGTGTCAAGTTCGAGGGCAAAGCAGCACGCAACAGGAGTGCAA
AACGACAATCAGCAAGAGACCAAAAGATGGA[]CGATGCTGGTGACA
CCTTGTATAATGGAGTTCAAGAGAATATGAATCATGCACCAGACTGGACCG{ACGCGT}ATCGGATTAAGAAG
AAGACTCATGCATACGCTATGCAATTTGGTAGGGAAGTACCAAAGACTGAAACACAGCGATCCTCACAATAC
TGGCACTTCTACGGTTTTGATCCAAAGATGTATGACTCAGTCGAATTCAAGGACATAGCAGCAAACTTCTCAG
TGCACCAGGATGCAAAGGCAATGGATTTGCAG[]TGGAAAATCGTTGGGATG
ATGAAGACTTCTTCGACGAGAAGATACCAAAGCGAGTTTTGGCCATCTTCAGGAAAGGAGACAAGGTTCGTG
AAGTTGCATTGGCACCTCACAAGCCAAACCAAGTCAACAAGCGTGGGCTACCTGTCGGACATGCTGATCAC
AGAGGAGAGTGGAGACAAACACAGCCTTCATTTGAAAAAGAAGTGTCGTACGAGAACAAATCAACTTTCGA
AGGTGCACGTTCACTTGATCATATCCATCAGAATCAAGTCATCCTCGTTGAAGACAATCAGCAGTTAAATGG
GCTAATAGTTGGGAACATACTCTTGGCGCCATATCATTTCACACGAGG[TATGAGGAACAGAGAGGAGAA]
[_], RT-PCR primer binding site; ,Nested-PCR primer binding site; { }, mutation site;
*표시된 primer set를 사용하면 양성대조구로부터의 오염여부 확인 가능
식물검역바이러스 검사법 ? 443

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