총 694페이지
573페이지 본문시작
다. 이러한 결과로 인해 우리는 Clavibacter californiensis sp.의 설립을 제안하게 되었
습니다. 11월 유형 균주 C55T(=CFBP 8216T=ATCC BAA-2691T)를 갖는 종으로 분류
됩니다. 또한 위에서 설명한 생물정보학 전략과 함께 이종상동 및 in silico 도트 플롯
분석을 통해 C. michiganensis subsp. chilensis 및 C. michiganensis subsp. phaseoli
의 상당한 연관성을 확인했습니다. 이러한 분석을 바탕으로 우리는 두 아종을 단일 분
류군으로 결합하고 Clavibacter phaseoli sp. nov.로 종 수준으로 승격시킬 것을 제안
합니다.
3. 원 문
Elevation of Clavibacter michiganensis subsp. californiensis to species level as Clavibacter
californiensis sp. nov., merging and re-classification of Clavibacter michiganensis subsp.
chilensis and Clavibacter michiganensis subsp. phaseoli as Clavibacter phaseoli sp. nov. based
on complete genome in silico analyses
Dario Arizala?, Shefali Dobhal, Anne M. Alvarez? and Mohammad Arif
The Gram-positive genus Clavibacter is currently divided into seven species ( Clavibacter
michiganensis , Clavibacter nebraskensis , Clavibacter capsici , Clavibacter sepedonicus ,
Clavibacter tessellarius , Clavibacter insidiosus and Clavibacter zhangzhiyongii ) and three
subspecies ( C. michiganensis subsp. californiensis , C. michiganensis subsp. chilensis and C.
michiganensis subsp. phaseoli ). Recent studies have indicated that the taxonomic rank of the
subspecies must be re-evaluated. In this research, we assessed the taxonomic position of the
three C. michiganensis subspecies and clarified the taxonomic nomenclature of other 75
Clavibacter strains. The complete genomes of the type strains of the three Clavibacter
subspecies, the type strain of C. tessellarius and C. nebraskensis A6096 were sequenced using
PacBio RSII technology. Application of whole-genome-based computational approaches such as
average nucleotide identity (ANI), digital DNA?DNA hybridization, multi-locus sequence analysis
of seven housekeeping genes (acnA, atpD, bipA, icdA, mtlD, recA and rpoB), a phylogenomic
tree reconstructed from 1?028 core genes, and ANI-based phylogeny provided sufficient
justification for raising C. michiganensis subsp. californiensis to the species level. These
results led us to propose the establishment of Clavibacter californiensis sp. nov. as a species
with its type strain C55T (=CFBP 8216T=ATCC BAA-2691T). Moreover, the orthologous and in
silico dot plot analyses, along with the above described bioinformatic strategies, revealed a
high degree of similarity between C. michiganensis subsp. chilensis and C. michiganensis
subsp. phaseoli . Based on these analyses, we propose that both subspecies be combined into
a single taxon and elevated to the species level as Clavibacter phaseoli sp. nov., with LPPA
982T (=?CECT 8144T=?LMG 27667T) as the type strain.
- 574 -
습니다. 11월 유형 균주 C55T(=CFBP 8216T=ATCC BAA-2691T)를 갖는 종으로 분류
됩니다. 또한 위에서 설명한 생물정보학 전략과 함께 이종상동 및 in silico 도트 플롯
분석을 통해 C. michiganensis subsp. chilensis 및 C. michiganensis subsp. phaseoli
의 상당한 연관성을 확인했습니다. 이러한 분석을 바탕으로 우리는 두 아종을 단일 분
류군으로 결합하고 Clavibacter phaseoli sp. nov.로 종 수준으로 승격시킬 것을 제안
합니다.
3. 원 문
Elevation of Clavibacter michiganensis subsp. californiensis to species level as Clavibacter
californiensis sp. nov., merging and re-classification of Clavibacter michiganensis subsp.
chilensis and Clavibacter michiganensis subsp. phaseoli as Clavibacter phaseoli sp. nov. based
on complete genome in silico analyses
Dario Arizala?, Shefali Dobhal, Anne M. Alvarez? and Mohammad Arif
The Gram-positive genus Clavibacter is currently divided into seven species ( Clavibacter
michiganensis , Clavibacter nebraskensis , Clavibacter capsici , Clavibacter sepedonicus ,
Clavibacter tessellarius , Clavibacter insidiosus and Clavibacter zhangzhiyongii ) and three
subspecies ( C. michiganensis subsp. californiensis , C. michiganensis subsp. chilensis and C.
michiganensis subsp. phaseoli ). Recent studies have indicated that the taxonomic rank of the
subspecies must be re-evaluated. In this research, we assessed the taxonomic position of the
three C. michiganensis subspecies and clarified the taxonomic nomenclature of other 75
Clavibacter strains. The complete genomes of the type strains of the three Clavibacter
subspecies, the type strain of C. tessellarius and C. nebraskensis A6096 were sequenced using
PacBio RSII technology. Application of whole-genome-based computational approaches such as
average nucleotide identity (ANI), digital DNA?DNA hybridization, multi-locus sequence analysis
of seven housekeeping genes (acnA, atpD, bipA, icdA, mtlD, recA and rpoB), a phylogenomic
tree reconstructed from 1?028 core genes, and ANI-based phylogeny provided sufficient
justification for raising C. michiganensis subsp. californiensis to the species level. These
results led us to propose the establishment of Clavibacter californiensis sp. nov. as a species
with its type strain C55T (=CFBP 8216T=ATCC BAA-2691T). Moreover, the orthologous and in
silico dot plot analyses, along with the above described bioinformatic strategies, revealed a
high degree of similarity between C. michiganensis subsp. chilensis and C. michiganensis
subsp. phaseoli . Based on these analyses, we propose that both subspecies be combined into
a single taxon and elevated to the species level as Clavibacter phaseoli sp. nov., with LPPA
982T (=?CECT 8144T=?LMG 27667T) as the type strain.
- 574 -
573페이지 본문끝
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