총 808페이지

2-2-1-04
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부 기
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부기1. Coating buffer
........................................................................................................................... 1.59g
2
3
NaHCO
......................................................................................................................... 2.93g
3
D.W ...................................................................................................................... to 1,000ml
adjusted pH 9.6±0.1, Store at 4℃
부기2. Washing buffer
Tween 20 ................................................................................................................... 0.50ml
PBS ...................................................................................................................... to 1,000ml
Phosphate buffered saline(PBS)
NaCl ............................................................................................................................... 8.00g
.......................................................................................................................... 0.20g
2
4
........................................................................................................................ 1.19g
2
4
KCl ................................................................................................................................. 0.20g
D.W ...................................................................................................................... to 1,000ml
adjusted pH 7.2±0.2
부기4. Blocking buffer
Bovine serum albumin ............................................................................................. 2.00g
PBS ......................................................................................................................... to 100ml
부기
5. Substrate solution(100ml
0.1M Citric acid(19.2g/L) .................................................................................... 24.30ml
0.2M Na
(28.4g/L) ....................................................................................... 25.70ml
2
D.W ............................................................................................................................ 50.00ml
OPD(o-Phenylenediamine) ................................................................................. 40.00mg
30% H
.................................................................................................................. 40.00㎕
2
2
부기6. Stopping solution
2.5M H
2
4

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