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sealed by Parafilm on the plant. Control plants were treated in the same manner with sterile
agar plugs. R. officinalis and T. vulgaris plants were flooded up to the collar with deionized
water for 24 h. All plants were maintained in a greenhouse at 22°C and watered when needed.
All inoculated plants showed root rot or leaf necrosis after 10 days, and control plants
remained healthy. The pathogen was reisolated from all infected plant tissues and confirmed
as P. nicotianae by molecular methods. P. nicotianae is a cosmopolitan pathogen (CABI 2021)
and was found on the given hosts in southern Europe. However, this is its first report in the
Czech Republic. Its introduction poses a potential risk for the growth of many ornamental and
agricultural plants in the country.
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agar plugs. R. officinalis and T. vulgaris plants were flooded up to the collar with deionized
water for 24 h. All plants were maintained in a greenhouse at 22°C and watered when needed.
All inoculated plants showed root rot or leaf necrosis after 10 days, and control plants
remained healthy. The pathogen was reisolated from all infected plant tissues and confirmed
as P. nicotianae by molecular methods. P. nicotianae is a cosmopolitan pathogen (CABI 2021)
and was found on the given hosts in southern Europe. However, this is its first report in the
Czech Republic. Its introduction poses a potential risk for the growth of many ornamental and
agricultural plants in the country.
- 608 -
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