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commercial ELISA test systems (Russian Potato Research Center, Russia).
In most samples (43 leaf samples), PVY was identified with other potato viruses, mainly
PVM and PVS. To type the PVY recombinant strains, samples with PVY monoinfection
were selected. ELISA detected 21 PVY monoinfection positive samples (14 Tavria and 7
Izolda). Serotype analysis using anti-rabbit polyclonal antibodies (Bioreba Ag, Switzerland)
specific for PVYO, PVYC, and PVYN serotypes identified the PVYO serotype in 14 Tavria
and 7 Izolda samples and the PVYN serotype in 2 Tavria and 2 Izolda samples. All 21
PVY-positive samples were tested for strain by reverse transcription PCR using
strain-specific primers described by Chikh Ali et al. (2010). The products of PCR analysis
showed bands characteristic of the recombinant N:O strain (853, 633, and 441 bp) in 17
samples and bands characteristic of the NTNa strain (1,307; 633; and 441 bp) in 4
samples.
After analysis of the leaf samples, PVY-infected plants were removed from the soil and
the tuber material was visually analyzed for signs of necrosis. Symptoms of tuber
necrosis were found in 80% of cases of infection with recombinant strains of PVYNTNa.
Symptoms of tuber necrosis were identical in both strains and caused damage to 35 to
50% of tubers on each plant of the Tavria and Isolda varieties, indicating the absence of
resistance to these strains. At present, many potato varieties have strain-specific
resistance to PVYO (Funke et al. 2017), but N:O and NTNa recombinant strains are the
most difficult to develop resistance to (Green et al. 2017). This is the first report of
Tavria and Izolda potatoes as susceptible hosts to recombinant strains of PVYN:O and
PVYNTNa. Over the past 5 years, recombinant strains PVYN:O and PVYNTNa were
introduced in two regions of Kazakhstan. Research on strategies to reduce the spread of
recombinant strains PVYN:O and PVYNTNa in Kazakhstan is needed.
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In most samples (43 leaf samples), PVY was identified with other potato viruses, mainly
PVM and PVS. To type the PVY recombinant strains, samples with PVY monoinfection
were selected. ELISA detected 21 PVY monoinfection positive samples (14 Tavria and 7
Izolda). Serotype analysis using anti-rabbit polyclonal antibodies (Bioreba Ag, Switzerland)
specific for PVYO, PVYC, and PVYN serotypes identified the PVYO serotype in 14 Tavria
and 7 Izolda samples and the PVYN serotype in 2 Tavria and 2 Izolda samples. All 21
PVY-positive samples were tested for strain by reverse transcription PCR using
strain-specific primers described by Chikh Ali et al. (2010). The products of PCR analysis
showed bands characteristic of the recombinant N:O strain (853, 633, and 441 bp) in 17
samples and bands characteristic of the NTNa strain (1,307; 633; and 441 bp) in 4
samples.
After analysis of the leaf samples, PVY-infected plants were removed from the soil and
the tuber material was visually analyzed for signs of necrosis. Symptoms of tuber
necrosis were found in 80% of cases of infection with recombinant strains of PVYNTNa.
Symptoms of tuber necrosis were identical in both strains and caused damage to 35 to
50% of tubers on each plant of the Tavria and Isolda varieties, indicating the absence of
resistance to these strains. At present, many potato varieties have strain-specific
resistance to PVYO (Funke et al. 2017), but N:O and NTNa recombinant strains are the
most difficult to develop resistance to (Green et al. 2017). This is the first report of
Tavria and Izolda potatoes as susceptible hosts to recombinant strains of PVYN:O and
PVYNTNa. Over the past 5 years, recombinant strains PVYN:O and PVYNTNa were
introduced in two regions of Kazakhstan. Research on strategies to reduce the spread of
recombinant strains PVYN:O and PVYNTNa in Kazakhstan is needed.
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