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2-2-2-12
<
부 기
>
부기
1.
세포증식용 배지
-MEM ........................................................................................................................ 430ml
Non-essential amino acid ............................................................................. 100 X 5ml
L-glutamin ........................................................................................................ 100 X 5ml
Sodium pyruvate ............................................................................................. 100 X 5ml
FBS(Gibco BRL 16000-044) .......................................................................... 10% 50ml
Antibiotic ........................................................................................................... 100 X 5ml
부기
2.
세포유지용 배지
(cell freezing medium)
Hybridoma ...................................................................................................... 300
만개이상
-MEM ............................................................................................................................ 7ml
DMSO .............................................................................................................................. 1ml
FBS ................................................................................................................................... 2ml
부기
3.
인산완충식염수
(phosphate buffered saline, PBS)
Sodium chloride ........................................................................................................... 8.0g
Potassium chloride ...................................................................................................... 0.2g
Disodium hydrogen orthophosphate(anhydrous) .............................................. 1.15g
Potassium dehydrogen ............................................................................................... 0.2g
D.W ........................................................................................................................... 1,000ml
최종
7.3
이되게 조정하여
15Lb
에서
15
분간 멸균 후 사용한다
부기4. Mounting buffer
Glycine .......................................................................................................................... 0.42g
Sodium hydroxide ........................................................................................................ 0.2g
Sodium chloride ......................................................................................................... 0.51g
Sodium azide ............................................................................................................... 0.03g
상기시약을 증류수
30ml
에 녹인 후
glycerine 70ml
을 첨가한다
<
부 기
>
부기
1.
세포증식용 배지
-MEM ........................................................................................................................ 430ml
Non-essential amino acid ............................................................................. 100 X 5ml
L-glutamin ........................................................................................................ 100 X 5ml
Sodium pyruvate ............................................................................................. 100 X 5ml
FBS(Gibco BRL 16000-044) .......................................................................... 10% 50ml
Antibiotic ........................................................................................................... 100 X 5ml
부기
2.
세포유지용 배지
(cell freezing medium)
Hybridoma ...................................................................................................... 300
만개이상
-MEM ............................................................................................................................ 7ml
DMSO .............................................................................................................................. 1ml
FBS ................................................................................................................................... 2ml
부기
3.
인산완충식염수
(phosphate buffered saline, PBS)
Sodium chloride ........................................................................................................... 8.0g
Potassium chloride ...................................................................................................... 0.2g
Disodium hydrogen orthophosphate(anhydrous) .............................................. 1.15g
Potassium dehydrogen ............................................................................................... 0.2g
D.W ........................................................................................................................... 1,000ml
최종
7.3
이되게 조정하여
15Lb
에서
15
분간 멸균 후 사용한다
부기4. Mounting buffer
Glycine .......................................................................................................................... 0.42g
Sodium hydroxide ........................................................................................................ 0.2g
Sodium chloride ......................................................................................................... 0.51g
Sodium azide ............................................................................................................... 0.03g
상기시약을 증류수
30ml
에 녹인 후
glycerine 70ml
을 첨가한다
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