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Comparative Study between the Automated MPN Method (TEMPO
CC) and
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the Standard Methods for Enumeration of Total Coliform Bacteria in Livestock
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Abstract£º
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To evaluate the applicability of a new automated coliform enumeration system(TEMPO
CC)
for the quantitative test of coliform bacteria contaminated in domestic livestock process
¨Þ
products, a total of 507 samples of livestock products were tested by methods of TEMPO
CC, the most probable number(MPN), and Petrifilm, rsepectively. The results of those three
methods were compared to each other. Of 507 samples of livestock products used in this
study, 217 samples were contaminated artificially with coliform bacteria and the rest(n=290)
¨Þ
were contaminated naturally. The results of the TEMPO
CC method for all samples were
equivalent to those obtained from the MPN method, except 8 samples. In addition, 496
samples(97.8%) out of 507 samples made agreement between the TEMPO method and the
¨Þ
Petrifilm method. The collelation coefficients between TEMPO
CC and MPN methods as
¨Þ
well as between TEMPO
CC and Petrifilm methods were above 0.9, and the slope and
intercept of the linear regression model was different in less than 1 value. In conclusion,
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there were statistically equivalent levels of performance between the TEMPO
CC and the
reference and alternative methods for the enumeration of coliform bacteria in livestock process
products in the study.
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