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축산식품 중 오염지표 세균의 정량검사법 개선에 관한 연구
연
Evaluation of the Automated MPN method (TEMPOⓡTVC) with Petrifilm
구
발표제목
method for Enumeration of Total Aerobic Bacteria in Livestock Products
성
과
Young Jo Kim, NohAReum, HaJeongYoon, Eun Jeong Heo, Hyun Jeong Park,
발표자
활
Hyun Jung Kim, Jin San Moon, Sung Hwan Wee
용
집
발표처
대한수의학회
발표시기
2011년 10월
수록잡지
(권, 호) 기재
Abstract：
As the importance of total quality approach in food production increase, microbial quality
indicator such as total aerobic bacteria play a significant role in HACCP plan and risk
assessment procedures to monitor the hygienic and commercial quality of food. Because
quality indicator sanalysisis routinely performed by a foodmicrobiology laboratory, microbial
testing process should be rapid, accurate and easy to use. In this study, we compared a new
®
TM
automated total viable counte numeration method (TEMPO
TVC) to Petrifilm
of standard
method in various livestock products.
Of total 347 samples,257 livestock products randomly selected in local retail stores and 87
livestock products artificially inoculated with E. coli ATCC 25922, Staphylococcus aureus
ATCC12868 were tested in this study. The test solution was made by adding 225 mL of
sterilized saline solution to 25 g of a sample and being homogenized with a Stomacher. And
then, 10-fold serial dilutions have been done by inoculating the reference strain of
1
2
3
4
5
6
7
10
,10
,10
,10
,10
,10
,10
,and10
.Fortheenumeration of total aerobic bacteria, Petrifilm and
®
TEMPO
TVC methods were used. For the Petrifilm method, the test solution was 10-fold
serial dilutions, and 1 ml of the test solution for each level was inoculated and absorbed to
TM
®
the Petrifilm
. In addition, the TEMPO
TVC was tested to calculate the number of total
aerobic bacteria according to the manual provided by its manufacturer. In the
®
TEMPO
system,the test solution was 10-fold serial dilutions. The serial dilution for each test
®
was automatically inserted to TEMPO
TVC cards through the TEMPO filler in the
®
TEMPO
system and incubated for 48 hours at 35°C. The number of total aerobic bacteria
®
was enumerated by the TEMPO
reader automatically. After the experimental data were
converted to log values, the degree of agreement was analyzed according to the CCFRA
Guideline N°29. and the agreement indicates the difference between the two methods is lower
than 1 log base 10. In addition, the correlation between two test methods was analyzed
The products that showed above 92% in the degree of agreement were in samples of hams,
jerky products, ground meat products, meat extract products, milks, cheeses, ice creams,
724
축산식품 중 오염지표 세균의 정량검사법 개선에 관한 연구
연
Evaluation of the Automated MPN method (TEMPOⓡTVC) with Petrifilm
구
발표제목
method for Enumeration of Total Aerobic Bacteria in Livestock Products
성
과
Young Jo Kim, NohAReum, HaJeongYoon, Eun Jeong Heo, Hyun Jeong Park,
발표자
활
Hyun Jung Kim, Jin San Moon, Sung Hwan Wee
용
집
발표처
대한수의학회
발표시기
2011년 10월
수록잡지
(권, 호) 기재
Abstract：
As the importance of total quality approach in food production increase, microbial quality
indicator such as total aerobic bacteria play a significant role in HACCP plan and risk
assessment procedures to monitor the hygienic and commercial quality of food. Because
quality indicator sanalysisis routinely performed by a foodmicrobiology laboratory, microbial
testing process should be rapid, accurate and easy to use. In this study, we compared a new
®
TM
automated total viable counte numeration method (TEMPO
TVC) to Petrifilm
of standard
method in various livestock products.
Of total 347 samples,257 livestock products randomly selected in local retail stores and 87
livestock products artificially inoculated with E. coli ATCC 25922, Staphylococcus aureus
ATCC12868 were tested in this study. The test solution was made by adding 225 mL of
sterilized saline solution to 25 g of a sample and being homogenized with a Stomacher. And
then, 10-fold serial dilutions have been done by inoculating the reference strain of
1
2
3
4
5
6
7
10
,10
,10
,10
,10
,10
,10
,and10
.Fortheenumeration of total aerobic bacteria, Petrifilm and
®
TEMPO
TVC methods were used. For the Petrifilm method, the test solution was 10-fold
serial dilutions, and 1 ml of the test solution for each level was inoculated and absorbed to
TM
®
the Petrifilm
. In addition, the TEMPO
TVC was tested to calculate the number of total
aerobic bacteria according to the manual provided by its manufacturer. In the
®
TEMPO
system,the test solution was 10-fold serial dilutions. The serial dilution for each test
®
was automatically inserted to TEMPO
TVC cards through the TEMPO filler in the
®
TEMPO
system and incubated for 48 hours at 35°C. The number of total aerobic bacteria
®
was enumerated by the TEMPO
reader automatically. After the experimental data were
converted to log values, the degree of agreement was analyzed according to the CCFRA
Guideline N°29. and the agreement indicates the difference between the two methods is lower
than 1 log base 10. In addition, the correlation between two test methods was analyzed
The products that showed above 92% in the degree of agreement were in samples of hams,
jerky products, ground meat products, meat extract products, milks, cheeses, ice creams,
724
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