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gloeosporioides (KSU-CgM17) and Colletotrichum siamense (KSU-CsM42). The pathogenicity was
tested on detached coffee leaves and green and red berries (Cao et al. 2019). For inoculation,
healthy leaves and berries were wounded with a sterilized needle, placed inside Petri dishes
containing moist filter paper, and then inoculated with a 10-μl droplet of conidial suspension
(106 spores/ml). Sterile distilled water was used as a negative control. Six replicates were
tested per isolate, and the experiment was repeated once. The inoculated coffee materials were
incubated at 25°C and 100% relative humidity for 8 days. Necrotic lesions developed on 100%
of the inoculated coffee materials after 6 days, whereas the negative controls were
asymptomatic. Koch’s postulates were fulfilled when typical colonies of these species were
successfully reisolated from the symptomatic tissues. These pathogens were previously reported
to affect coffee in Vietnam (Nguyen et al. 2010), China (Cao et al. 2019), and Puerto Rico
(Serrato-Diaz et al. 2020). To our knowledge, this is the first record of C. gloeosporioides and
C. siamense causing coffee anthracnose disease in Saudi Arabia. Further studies on the
epidemiology of anthracnose disease on arabica coffee plantations as well as effective
strategies for managing this disease are needed.
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tested on detached coffee leaves and green and red berries (Cao et al. 2019). For inoculation,
healthy leaves and berries were wounded with a sterilized needle, placed inside Petri dishes
containing moist filter paper, and then inoculated with a 10-μl droplet of conidial suspension
(106 spores/ml). Sterile distilled water was used as a negative control. Six replicates were
tested per isolate, and the experiment was repeated once. The inoculated coffee materials were
incubated at 25°C and 100% relative humidity for 8 days. Necrotic lesions developed on 100%
of the inoculated coffee materials after 6 days, whereas the negative controls were
asymptomatic. Koch’s postulates were fulfilled when typical colonies of these species were
successfully reisolated from the symptomatic tissues. These pathogens were previously reported
to affect coffee in Vietnam (Nguyen et al. 2010), China (Cao et al. 2019), and Puerto Rico
(Serrato-Diaz et al. 2020). To our knowledge, this is the first record of C. gloeosporioides and
C. siamense causing coffee anthracnose disease in Saudi Arabia. Further studies on the
epidemiology of anthracnose disease on arabica coffee plantations as well as effective
strategies for managing this disease are needed.
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