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과제명
국내 돼지생식기호흡기증후군의 감시 프로그램 및 예방약 개발에 관한 연구
연
구
A survey of porcine reproductive and respiratory syndrome virus in wild boar
발표제목
성
populations in Korea
과
발표자
최은진
활
발표처
국제동물전문가학회(CRWAD)
발표시기
2011년 12월
용
집
수록잡지
세계 동물질병전문가회의 (CRWAD) 초록집
Abstract：
Purpose: No information is currently available on pocine reproductive and respiratory
syndrome virus(PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the
status of PRRS in wild boars was investigated.
Methods: Blood samples were collected form 267 wild boars from 8 provinces in Korea. The
samples were tested for antibodies and antigens to PRRSV using a commercial enzyme-linked
immunosorbent assay (ELISA) kit and a reverse transcriptase-polymerase chain reaction
(RT-PCR) assay to detect PRRSV and to differentiate two genotypes.
Results: Four (1.5%) and 8 (3.0%) of the samples tested were positive for PRRSV antibodies
and antigens, respectively. Of he virus positive samples, 3 and 5 samples were types as
European (EU, type1) and North American (NA, type2) viruses, respectively. Two amplicons
(one from type1 PRRSV ORF7 nucleotide sequences of wild boar had identities between 96.1
and 98.4% with those of domestic pig-derived PRRS viruses in Korea. The type 2 PRRSV
ORF7 sequences of wild boar had identities of 100% with those of PRRS virus VR2332
strain. The homologies of deduced amino acid sequences of ORF7 between wild boar-type 1
virus and Lelystad virus strain was 92.2%.
Conclusions: These results show that PRRSVs are present in wild boars in Korea, and
therefore, effective PRRSV surveillance in wild boar population might be useful in desease
control.
798
국내 돼지생식기호흡기증후군의 감시 프로그램 및 예방약 개발에 관한 연구
연
구
A survey of porcine reproductive and respiratory syndrome virus in wild boar
발표제목
성
populations in Korea
과
발표자
최은진
활
발표처
국제동물전문가학회(CRWAD)
발표시기
2011년 12월
용
집
수록잡지
세계 동물질병전문가회의 (CRWAD) 초록집
Abstract：
Purpose: No information is currently available on pocine reproductive and respiratory
syndrome virus(PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the
status of PRRS in wild boars was investigated.
Methods: Blood samples were collected form 267 wild boars from 8 provinces in Korea. The
samples were tested for antibodies and antigens to PRRSV using a commercial enzyme-linked
immunosorbent assay (ELISA) kit and a reverse transcriptase-polymerase chain reaction
(RT-PCR) assay to detect PRRSV and to differentiate two genotypes.
Results: Four (1.5%) and 8 (3.0%) of the samples tested were positive for PRRSV antibodies
and antigens, respectively. Of he virus positive samples, 3 and 5 samples were types as
European (EU, type1) and North American (NA, type2) viruses, respectively. Two amplicons
(one from type1 PRRSV ORF7 nucleotide sequences of wild boar had identities between 96.1
and 98.4% with those of domestic pig-derived PRRS viruses in Korea. The type 2 PRRSV
ORF7 sequences of wild boar had identities of 100% with those of PRRS virus VR2332
strain. The homologies of deduced amino acid sequences of ORF7 between wild boar-type 1
virus and Lelystad virus strain was 92.2%.
Conclusions: These results show that PRRSVs are present in wild boars in Korea, and
therefore, effective PRRSV surveillance in wild boar population might be useful in desease
control.
798
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