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중 하나와 비교하여 3개의 돌연변이를 나타냈다. 서로 다른 포도나무는 상대적으로 열
악하고 발육 부진을 보였기 때문에 초기에 샘플링되었지만 GRVFV 및/또는 GRGV 외에
HTS 분석에 따르면 홉 스턴트 바이로이드, 포도나무 황색 스펙클 바이로이드 1, 포도
나무 루페스트리스 줄기 구멍 바이러스, 새로운 nepovirus(#4), 포도나무 잎사귀 관련
바이러스 2 및 포도나무 Pinot gris 바이러스(#5b)이므로 여기에 보고된 결과는 GRGV
또는 GRVFV의 잠재적 병원성에 대한 새로운 빛을 비추지 않습니다.
이것은 포르투갈의 포도나무에서 GRGV 및 GRVFV의 첫 번째 보고서입니다.
3. 원 문
First Report of Grapevine Red Globe Virus (GRGV) and Grapevine Rupestris Vein Feathering
Virus (GRVFV) Infecting Grapevine (Vitis vinifera) in Portugal
T. Candresse, C. Faure, and A. Marais
Grapevine red globe virus (GRGV) and Grapevine rupestris vein feathering virus (GRVFV) are
relatively recently described grapevine-infecting viruses that respectively belong to the genera
Maculavirus and Marafivirus in the family Tymoviridae (Martelli 2014). Owing to their rather
recent description, limited information on their biology, molecular variability, and geographic
distribution is available. Both viruses are apparently completely or largely asymptomatic in
European grapevine and have likely been overlooked in a wide range of situations (Martelli
2014). According to sequences in GenBank, GRGV has been identified in Asia (Iran, Japan, and
China), the Americas (United States and Brazil) and Europe (Spain, France, Slovenia, Hungary,
Czech Republic, and Germany). GRVFV has been reported from the same countries and also
from Oceania (New Zealand and Australia) and a range of other countries, including India,
Pakistan, and South Korea in Asia; Canada in North America; and Switzerland, Slovakia, Italy,
and Russia in Europe. Evidence for the presence of GRGV and GRVFV in grapevine plants
from northern Portugal (variety(ies) unknown) was obtained through the bioinformatic analysis
(Candresse et al. 2013) of RNASeq Illumina sequencing data obtained from the total RNA
isolated from the phloem scrapings of five grapevine samples collected in different plots in
2016 (Candresse et al. 2018). Following grapevine genome (GCA_000003745.2) subtraction, contig
assembly, and BLASTn- and BLASTX-based contig annotation using CLC Genomics Workbench,
two plants, #4 and #5b, yielded contigs representing near complete GRGV genomes. The #4
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악하고 발육 부진을 보였기 때문에 초기에 샘플링되었지만 GRVFV 및/또는 GRGV 외에
HTS 분석에 따르면 홉 스턴트 바이로이드, 포도나무 황색 스펙클 바이로이드 1, 포도
나무 루페스트리스 줄기 구멍 바이러스, 새로운 nepovirus(#4), 포도나무 잎사귀 관련
바이러스 2 및 포도나무 Pinot gris 바이러스(#5b)이므로 여기에 보고된 결과는 GRGV
또는 GRVFV의 잠재적 병원성에 대한 새로운 빛을 비추지 않습니다.
이것은 포르투갈의 포도나무에서 GRGV 및 GRVFV의 첫 번째 보고서입니다.
3. 원 문
First Report of Grapevine Red Globe Virus (GRGV) and Grapevine Rupestris Vein Feathering
Virus (GRVFV) Infecting Grapevine (Vitis vinifera) in Portugal
T. Candresse, C. Faure, and A. Marais
Grapevine red globe virus (GRGV) and Grapevine rupestris vein feathering virus (GRVFV) are
relatively recently described grapevine-infecting viruses that respectively belong to the genera
Maculavirus and Marafivirus in the family Tymoviridae (Martelli 2014). Owing to their rather
recent description, limited information on their biology, molecular variability, and geographic
distribution is available. Both viruses are apparently completely or largely asymptomatic in
European grapevine and have likely been overlooked in a wide range of situations (Martelli
2014). According to sequences in GenBank, GRGV has been identified in Asia (Iran, Japan, and
China), the Americas (United States and Brazil) and Europe (Spain, France, Slovenia, Hungary,
Czech Republic, and Germany). GRVFV has been reported from the same countries and also
from Oceania (New Zealand and Australia) and a range of other countries, including India,
Pakistan, and South Korea in Asia; Canada in North America; and Switzerland, Slovakia, Italy,
and Russia in Europe. Evidence for the presence of GRGV and GRVFV in grapevine plants
from northern Portugal (variety(ies) unknown) was obtained through the bioinformatic analysis
(Candresse et al. 2013) of RNASeq Illumina sequencing data obtained from the total RNA
isolated from the phloem scrapings of five grapevine samples collected in different plots in
2016 (Candresse et al. 2018). Following grapevine genome (GCA_000003745.2) subtraction, contig
assembly, and BLASTn- and BLASTX-based contig annotation using CLC Genomics Workbench,
two plants, #4 and #5b, yielded contigs representing near complete GRGV genomes. The #4
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