총 307페이지

11.
동물세포 조직배양
··········································································································· 140
12.
단클론 항체
······················································································································· 161
13. 형광항체법 ························································································································
14.
효소면역 단층세포측정법
······························································································· 174
15.
효소결합면역항체법
········································································································· 175
16.
면역크로마토그라피 신속진단킷트 제작
····································································· 182
17.
최소 치사량과 평균치사시간을 구하는 방법
····························································· 189
18. 바이러스의 부화계란접종 ································································································ 190
19. Plaque assay (or Virus cloning) ·············································································· 197
분자미생물학적 실험기법
1. Whole blood, buffy coat
혹은
cultured cells
로부터
추출
························ 201
2. DNA extraction for PCR ······························································································· 203
3. RNA
의 분리와 정제
··········································································································· 204
4. DNA preparation from
E. coli ····················································································· 210
5. Transformation ·················································································································· 211
6. Rapid isolation of plasmid DNA using Sephacryl column ································· 212
7. Transfection ·······················································································································
8. cDNA Synthesis ················································································································ 215
9.
종합효소연쇄반응
(PCR) ···································································································· 216
10. Multiplex one-step RT-PCR ····················································································· 228
11. DNA
전기영동을 위한
agarose gel
제조
································································· 229
12. Sequencing gel electrophoresis ················································································ 231
13. DNA Sequencing ············································································································ 233
14. Western blotting ············································································································· 235
15. Immuno-Dot ····················································································································· 242
16. Electro immuno blotting ······························································································ 243
17. Apoptosis assay ············································································································· 245
동물의 질병진단
1.
가축질병의 진단
················································································································· 248
<
>
동물별 임신기간
2.
수산 양식어류질병의 진단
······················································································· 291
- ii -

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