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Hypothesis
Duringdenaturationandrenaturation,PrP
Sc
refoldedPrP
likeconformation,whichisnolonger
C
infectious.
Experiments
1.IP
2.Modifiedcervidprioncellassay(CPCA)
ModifiedCervidPrionCellAssay(CPCA)
ELISpotplate
Plate20,000cells/well
20,000cells/well
Putinfectedmaterial
RegularCPCA
p1 p2 p3
Coatplate
w/infectedmaterial
Plate20,000cells/well
ModifiedCPCA
Immunodetection
Gdn.iso
ProteinaseK
50¢ªC
- 150 -
Duringdenaturationandrenaturation,PrP
Sc
refoldedPrP
likeconformation,whichisnolonger
C
infectious.
Experiments
1.IP
2.Modifiedcervidprioncellassay(CPCA)
ModifiedCervidPrionCellAssay(CPCA)
ELISpotplate
Plate20,000cells/well
20,000cells/well
Putinfectedmaterial
RegularCPCA
p1 p2 p3
Coatplate
w/infectedmaterial
Plate20,000cells/well
ModifiedCPCA
Immunodetection
Gdn.iso
ProteinaseK
50¢ªC
- 150 -
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