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UseofrecombinantApxIVinserodiagnosisofActinobacilluspleuropneumoniaeinfections,
developmentandprevalidationoftheApxIVELISA
Fig.CalibrationoftheApxIV-ELISA.Openbarsrepresentthefrequencyof
Fig. KineticsofApxIVsero-conversionofapig
ELISAtitersobtainedwith99serafromanimalsoriginatingfromA.
infectedwithA.pleuropneumoniae.Upper
pleuropneumoniae-negativefarmsJ,R,T,U.Blackbarsrepresentthe
panel:immunoblotscontainingpurified,
frequencyofELISAtitersobtainedwithserafromtotally80pigsthatwere
recombinantApxIVN¡Ç-Hisreactedwithserum
infectedbyA.pleuropneumoniae.
thatwastakensequentiallyatdays?5,7,14,
20,28,35,42,49,and55postinfection(p.i.)
withA.pleuropneumoniaeserotype9strain
4915.Themolecularmassscaleisindicatedon
A.Dreyfuset.al.VeterinaryMicrobiology,Volume99,2004,227?238
theleft.Lowerpanel:ApxIV-ELISAtitersofthe
samesera.
C532E
developmentandprevalidationoftheApxIVELISA
Fig.CalibrationoftheApxIV-ELISA.Openbarsrepresentthefrequencyof
Fig. KineticsofApxIVsero-conversionofapig
ELISAtitersobtainedwith99serafromanimalsoriginatingfromA.
infectedwithA.pleuropneumoniae.Upper
pleuropneumoniae-negativefarmsJ,R,T,U.Blackbarsrepresentthe
panel:immunoblotscontainingpurified,
frequencyofELISAtitersobtainedwithserafromtotally80pigsthatwere
recombinantApxIVN¡Ç-Hisreactedwithserum
infectedbyA.pleuropneumoniae.
thatwastakensequentiallyatdays?5,7,14,
20,28,35,42,49,and55postinfection(p.i.)
withA.pleuropneumoniaeserotype9strain
4915.Themolecularmassscaleisindicatedon
A.Dreyfuset.al.VeterinaryMicrobiology,Volume99,2004,227?238
theleft.Lowerpanel:ApxIV-ELISAtitersofthe
samesera.
C532E
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