총 27페이지

베트남-한국간ODA사업관련돼지유행성설사등바이러스질병진단교육
Interpretation
????ExpectedTGEPCRproductsize:755bp
????ExpectedPEDPCRproductsize:525bp
Fig1.ElectrophoresisofPCRproductbyi-TGEV/PEDVRT-PCRPremixKit
LaneM:100bpmolecularladder(iNtRONBiotechnology)
Lane1~4:TGE/PEDpositivesample(10folddilution)
LaneN:Negativecontrol
Troubleshooting
Trouble
Cause
Solutions
Have little extraction
? Use a Microconcentrator to
The amount of virus in the sample is small
concentrate the sample
? Check whether reagents were
The reagent was not used or the test
used correctly and the test
method was not followed
method
The RNA is broken
? Prepare the sample as soon as
possible
or
add
RNase
inhibitor to the sample
There are large amount of samples ( Using
? Adhere to the sample volume ,
a tissue samples )
and for tissue samples make
up the homogenate
RT-PCR results are not good
? After using Washing Buffer B,
Ethanol removal was not perfect
centrifuge at maximum rpm to
remove ethanol
Difficult to interpret results
? Reduce amount of template by
The amount of RNA is excessive
due to non-specific bands
1/10 dilution and reacts again
ODAprojectbetweenAPQA(Korea)andNCVD(Vietnam)
｜26
-TrainingcourseforPorcineEpidemicDiarrheaandviraldisease

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