총 141페이지
140페이지 본문시작
강현미, 이애리, 주이석, 문진산, 임숙경, 위성환,
발표자
발표시기
김옥경, 김말란
2001. 5. 23
발표제목
Distribution of methicillin-resistant and enterotoxin-producing Staphylococcus aureus
from mastitic milk of dairy cows in Korea
발표기관 미국미생물학회
수록잡지 미국미생물학회101st General Meeting 초록집
Abstract : Mastitis is the most costly disease in dairy cows. Staphylococcus aureus (S. aureus) is one
of major pathogen of bovine mastitis and 75% of them are approximately resistant to penicillin or
ampicillin, a part of them are also resistant to methicillin. As well enterotoxin-producing S. aureus is a
common cause of food poisoning. Raw milk containing enterotocigenic S. aureus may constitute a health
hazard to consumer. Therefore, this study was investigated the distribution of methicillin resistant S.
aureus (MRSA) using multiplex PCR and broth dilution minimal inhibitory concentration (MIC) and
determined the types on enterotoxin-producing S. aureus by reversed passive latex agglutination kit from
bovine mastitic milk of 964 dairy farms from September 1999 to August 2000. Multiplex PCR was carried
out to detect the coagulase gene (pathogenic of S. aureus) and mec A gene (characteristically encoding
for methicillin resistance in Staphylococci). 703 Strains of S. aureus in the 5,902 quarters or composite
milk were isolated from bovine mastitic milk of 175 dairy farms(18.3%). Of the 703 S. aureus, 179 strains
were determined the MRSA. When isolates with oxacillin MICs≥4㎍/㎖ were classified as methicillin
resistant, 17(9.5%) resistant and 162(90.5%) susceptible strains were detected. The mec A gene was found
with 13 of the 17 resistant strains, and mec A gene expression rate was higher in high MICs≥16㎍/㎖.
Although, 51 strains(31.5%) in the 162 of methicillin susceptible strains produced the enterotoxin, 14
strains(82.3%) in the 17 strains of MRSA produced enterotoxin A, B, C or D. The toxin types were
presented in the order of A〉C〉B〉D. The presence of MRSA suggests that epidemic MRSA clones
may be spread between different dairy farms. Therefore, use of appropriate antibiotics and control of
enterotoxigenic S. aureus are needed. Further studies will carry out molecular epidemiological relationship
of MRSA or enterotoxigenic MRSA.
발표자
발표시기
김옥경, 김말란
2001. 5. 23
발표제목
Distribution of methicillin-resistant and enterotoxin-producing Staphylococcus aureus
from mastitic milk of dairy cows in Korea
발표기관 미국미생물학회
수록잡지 미국미생물학회101st General Meeting 초록집
Abstract : Mastitis is the most costly disease in dairy cows. Staphylococcus aureus (S. aureus) is one
of major pathogen of bovine mastitis and 75% of them are approximately resistant to penicillin or
ampicillin, a part of them are also resistant to methicillin. As well enterotoxin-producing S. aureus is a
common cause of food poisoning. Raw milk containing enterotocigenic S. aureus may constitute a health
hazard to consumer. Therefore, this study was investigated the distribution of methicillin resistant S.
aureus (MRSA) using multiplex PCR and broth dilution minimal inhibitory concentration (MIC) and
determined the types on enterotoxin-producing S. aureus by reversed passive latex agglutination kit from
bovine mastitic milk of 964 dairy farms from September 1999 to August 2000. Multiplex PCR was carried
out to detect the coagulase gene (pathogenic of S. aureus) and mec A gene (characteristically encoding
for methicillin resistance in Staphylococci). 703 Strains of S. aureus in the 5,902 quarters or composite
milk were isolated from bovine mastitic milk of 175 dairy farms(18.3%). Of the 703 S. aureus, 179 strains
were determined the MRSA. When isolates with oxacillin MICs≥4㎍/㎖ were classified as methicillin
resistant, 17(9.5%) resistant and 162(90.5%) susceptible strains were detected. The mec A gene was found
with 13 of the 17 resistant strains, and mec A gene expression rate was higher in high MICs≥16㎍/㎖.
Although, 51 strains(31.5%) in the 162 of methicillin susceptible strains produced the enterotoxin, 14
strains(82.3%) in the 17 strains of MRSA produced enterotoxin A, B, C or D. The toxin types were
presented in the order of A〉C〉B〉D. The presence of MRSA suggests that epidemic MRSA clones
may be spread between different dairy farms. Therefore, use of appropriate antibiotics and control of
enterotoxigenic S. aureus are needed. Further studies will carry out molecular epidemiological relationship
of MRSA or enterotoxigenic MRSA.
140페이지 본문끝
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