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과 제 명 아보바이러스성 기립불능우의 주요 원인체 분석 및 병리학적 병변조사
논
Genetic and pathogenic characterization of Akabane viruses isolated from
문
발표제목
cattle with encephalomyelitis in Korea
게
재
발 표 자 Oem JK, Yoon HJ, Kim HR, Roh IS, Lee KH, Lee OS, Bae YC.
?
S
수록잡지 Veterinary Microbiology 158(3-4) : 259-266
발표시기
2012년 8월
C
I
Abstract :
?
A large-scale outbreak of Akabane viral encephalomyelitis in cattle was reported in the
southern part of Korea in 2010. Fifteen Akabane virus(AKAV) strains were isolated
from the brain and spinal cord samples by using BHK-21 and/or HmLu-1 cells. To
examine the genetic relationships and characteristics of the isolates, nucleotide
sequences of the S, M, and L segments of the 15 isolates were determined and
analyzed. Complete sequence analysis of the 15 AKAV isolates showed 99.9-100%
amino acid identities, indicating that the 15 isolates originated from a single strain. The
S and M RNA segments of a representative isolate(AKAV-7/SKR/2010) were also
compared with the segments of representative reference sequences. This
AKAV-7/SKR/2010 strain showed the highest identity with the Iriki and KM-1/Br/06
strains. Neighbor-joining phylogenetic trees of S and M RNA segments were
constructed. Four representative AKAV isolates were classified into subgroup Ia, which
contains the Iriki and KM-1/Br/06 strains recognized to cause encephalomyelitis in
calves and adult cattle in Japan. Moreover, experimental intraperitoneal infection was
performed using the AKAV-7/SKR/2010 and AKAV-17/SKR/2010 strains to assess
pathogenesis in suckling mice. The 2 isolates, genetically related to the Iriki strain,
were neurovirulent and caused neurological signs in suckling mice. In contrast, the
93FMX strain and the K0505 strain, related to the OBE-1 strain, were avirulent in mice.
The present results indicate that these isolates most likely had originated from the Iriki
strain and are closely related to the Iriki strain both genetically and pathogenically.
761
논
Genetic and pathogenic characterization of Akabane viruses isolated from
문
발표제목
cattle with encephalomyelitis in Korea
게
재
발 표 자 Oem JK, Yoon HJ, Kim HR, Roh IS, Lee KH, Lee OS, Bae YC.
?
S
수록잡지 Veterinary Microbiology 158(3-4) : 259-266
발표시기
2012년 8월
C
I
Abstract :
?
A large-scale outbreak of Akabane viral encephalomyelitis in cattle was reported in the
southern part of Korea in 2010. Fifteen Akabane virus(AKAV) strains were isolated
from the brain and spinal cord samples by using BHK-21 and/or HmLu-1 cells. To
examine the genetic relationships and characteristics of the isolates, nucleotide
sequences of the S, M, and L segments of the 15 isolates were determined and
analyzed. Complete sequence analysis of the 15 AKAV isolates showed 99.9-100%
amino acid identities, indicating that the 15 isolates originated from a single strain. The
S and M RNA segments of a representative isolate(AKAV-7/SKR/2010) were also
compared with the segments of representative reference sequences. This
AKAV-7/SKR/2010 strain showed the highest identity with the Iriki and KM-1/Br/06
strains. Neighbor-joining phylogenetic trees of S and M RNA segments were
constructed. Four representative AKAV isolates were classified into subgroup Ia, which
contains the Iriki and KM-1/Br/06 strains recognized to cause encephalomyelitis in
calves and adult cattle in Japan. Moreover, experimental intraperitoneal infection was
performed using the AKAV-7/SKR/2010 and AKAV-17/SKR/2010 strains to assess
pathogenesis in suckling mice. The 2 isolates, genetically related to the Iriki strain,
were neurovirulent and caused neurological signs in suckling mice. In contrast, the
93FMX strain and the K0505 strain, related to the OBE-1 strain, were avirulent in mice.
The present results indicate that these isolates most likely had originated from the Iriki
strain and are closely related to the Iriki strain both genetically and pathogenically.
761
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