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과 제 명 가축의 중독성 질병 발생 상황 조사 및 진단체계 구축 연구
논
Pathogenicity characterization of a bovine triple reassortant
문
발표제목
rotavirus in calves and piglets
게
재
Hyun-Jeong Kim, Jun-Gyu Park, Mia Madel Alfajaro, Deok-Song Kim, Myra
?
발 표 자
Hosmillo, Kyu-Yeol Son, Ju-Hwan Lee, You-Chan Bae, Sang-Ik Park, Mun-Il
S
Kang, Kyoung-Oh Cho
C
I
수록잡지 Veterinary microbiology 159(1-2) : 11-22
발표시기
2012년 9월
?
Abstract :
Rotaviruses are important human and animal pathogens with high impact on public
health and livestock industry. There is little evidence about the cross-species
pathogenicity and extra-intestinal infections of animal and human reassortant rotaviruses,
particularly based on all 11 genotyping data. In this study, the bovine triple reassortant
KJ56-1 strain harboring two bovine-like genome segments, eight porcine-like genome
segments, and one human-like genome segment was used to evaluate the cross-species
pathogenicity in its parent species, calves and piglets, and to determine its abilities of
causing viremia and extra-intestinal tropisms in piglets. The KJ56-1 strain isolated from
a calf diarrhea fecal sample replicated without causing diarrhea and severe intestinal
pathology in calves. However, piglets inoculated with this strain showed persistent
severe diarrhea and marked intestinal pathology. By SYBR Green real-time RT-PCR,
viral RNA was detected in the sera, mesenteric lymph node, lung, liver, choroid
plexus, and cerebrospinal fluid in the experimental piglets. An immunofluorescence
assay confirmed viral replication in these extra-intestinal organs and tissues. These
results indicated that the bovine triple reassortant KJ56-1 strain was virulent to piglets
but not to calves. Our data also demonstrated that the reassortant rotaviruses had the
ability to spread to the bloodstream from the gut, enter and amplify in the mesenteric
lymph node, and disseminate to the extra-intestinal organs and tissues.
763
논
Pathogenicity characterization of a bovine triple reassortant
문
발표제목
rotavirus in calves and piglets
게
재
Hyun-Jeong Kim, Jun-Gyu Park, Mia Madel Alfajaro, Deok-Song Kim, Myra
?
발 표 자
Hosmillo, Kyu-Yeol Son, Ju-Hwan Lee, You-Chan Bae, Sang-Ik Park, Mun-Il
S
Kang, Kyoung-Oh Cho
C
I
수록잡지 Veterinary microbiology 159(1-2) : 11-22
발표시기
2012년 9월
?
Abstract :
Rotaviruses are important human and animal pathogens with high impact on public
health and livestock industry. There is little evidence about the cross-species
pathogenicity and extra-intestinal infections of animal and human reassortant rotaviruses,
particularly based on all 11 genotyping data. In this study, the bovine triple reassortant
KJ56-1 strain harboring two bovine-like genome segments, eight porcine-like genome
segments, and one human-like genome segment was used to evaluate the cross-species
pathogenicity in its parent species, calves and piglets, and to determine its abilities of
causing viremia and extra-intestinal tropisms in piglets. The KJ56-1 strain isolated from
a calf diarrhea fecal sample replicated without causing diarrhea and severe intestinal
pathology in calves. However, piglets inoculated with this strain showed persistent
severe diarrhea and marked intestinal pathology. By SYBR Green real-time RT-PCR,
viral RNA was detected in the sera, mesenteric lymph node, lung, liver, choroid
plexus, and cerebrospinal fluid in the experimental piglets. An immunofluorescence
assay confirmed viral replication in these extra-intestinal organs and tissues. These
results indicated that the bovine triple reassortant KJ56-1 strain was virulent to piglets
but not to calves. Our data also demonstrated that the reassortant rotaviruses had the
ability to spread to the bloodstream from the gut, enter and amplify in the mesenteric
lymph node, and disseminate to the extra-intestinal organs and tissues.
763
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