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Establishment of a Cell Line Persistently Infected with Chronic Wasting
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Disease Prions
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Hyo-Jin Kim, Dong-Seob Tark, Yoon-Hee Lee, Min-Jeong Kim, Won-YongLee,
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In-SooCho, Hyun-Joo Sohn, Takashi Yokoyama
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¼ö·ÏÀâÁö The Journal of Veterinary Medical Science 74(10) : 1377-1380 ¹ßÇ¥½Ã±â
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Abstract :
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Elk prion protein(PrP
)has been confirmed capable of rendering rabbit epithelial RK13
cells permissive to temporal infection by chronic wasting disease(CWD) prions. The
present study satisfactorily generated persistently CWD prion affected RK13
C
cells(RKC1-11) using elk PrP
expressing cells(elkRK13) that were generated via the
lentiviral expression system with high efficiency. The elk RK13 cells are permissive to
Sc
accumulation of abnormal isoforms of prion protein(PrP
)resulting from CWD prions up
to 75 serial passages thus far. This novel prion affected cell line will help facilitate
investigation of the molecular basis of CWD prion pathogenesis and confirmation of
CWD prion infectivity in vitro.
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