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논
문
Gene expression profile of a persistently chronic wasting disease (CWD)
발표제목
게
prion-infected RK13 cell line
재
Hyo-Jin Kim, Won-Yong Lee, Min-Jeong Kim, Dong-Seob Tark, In-Soo Cho,
발 표 자
?
비
Hyun-Joo Sohn, Yoon-Hee Lee
S
수록잡지 한국수의공중보건학회지 36(4), 186-195
발표시기
2012년 12월
C
I
?
Abstract :
Chronic wasting disease (CWD) is a neurodegenerative disorder in cervids and a member
of the transmissible spongiform encephalopathies (TSEs), also known as prion diseases. We
previously generated a persistently CWD prion infected RK13 cell line (RKC1-11) using elk
PrPC expressing cells (elkRK13) that were generated with the lentiviral expression system.
To investigate the differentially expressed (DE) genes involved in prion infection at the
cellular level, we performed microarray analysis and identified the DE genes between
CWD-infected (RKC1-11) and non-infected cells (elkRK13). Collectively, 88 genes were
found to be differentially expressed (42 genes upregulated > 2-fold; 46 genes
downregulated < 0.5-fold); additionally, 10 up- and 8 downregulated genes agreed with the
results of the qRT-PCR. Among these genes, we chose 8 DE genes associated with cell
growth, signal transduction, transport, immune response and apoptosis based on gene
function analysis for further analysis. The expression of the selected genes was further
validated using an animal model in normal- and CWD-infected TgElk mice showing clinical
signs at 185 dpi. These identified DE genes in both the in vitro and in vivo model could
help in understanding the diagnosis and pathogenesis of prion diseases.
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