총 2151페이지

과 제 명 변형 프리온 증폭기법에 관한 연구
연
Sc
PrP
detection in affected tissues and blood of mice infected with
구
발표제목
murine-adapted bovine spongiform encephalopathy strain 301C
성
과
Yoon-Hee Lee, Hyun-Joo Sohn, Min-Jeong Kim, Hyo-Jin Kim, Won-Yong Lee,
발 표 자
Young-Pyo Choi, Dong-Seob Tark, Yi-Seok Joo, Chang-Hee Kweon,
활
In-Soo Cho
용
집
수록잡지 한국수의공중보건학회지 36(2) : 81-88
발표시기
2012년 6월
Abstract :
Sc
The sensitivities of PrP
detection methods, western blotting(WB), immunohistochemistry(IHC)
and protein misfolding cyclic amplification(PMCA) techniques were compared from
Sc
brains, spleens and blood of mice challenged with PrP
of murine-adapted BSE strain
Sc
301C. PrP
was detected in the spleen from 30 dpi by IHC and at 60 dpi by WB. At
Sc
30 dpi, disease-specific signals of PrP
was observed in only two follicles of a single
Sc
spleen. PrP
was detected in spleen at 10 dpi with PMCA after 5 rounds of
amplification. Clinical signs were obviously shown from 240 dpi, and coincided with
Sc
first detection of PrP
in brains by WB, IHC and PMCA after one round amplification.
Sc
In addition, PrP
was also detected in blood at 60, 180 and 240 dpi with PMCA after
5 rounds of amplification. The FDC-M1 epitope, which appears in immature FDCs, and
Sc
were detected in follicles first at 30 dpi, whilst the FDC-M2 epitope of mature
PrP
Sc
FDCs was detected at 60 dpi. More FDC-M2 epitope and PrP
were detected in
follicles as disease progressed. The CD21/35 epitope is expressed on both FDCs and
germinal center B cells. The pattern of CD21/35 expressing cells was similar to but
less dominant than that of FDCs
888

현재 포커스의 아래내용들은 동일한 컨텐츠를 가지고 페이지넘김 효과및 시각적 효과를 제공하는 페이지이므로 스크린리더 사용자는 여기까지만 낭독하시고 위의 페이지이동 링크를 사용하여 다음페이지로 이동하시기 바랍니다.