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과 제 명 조제 유류 중 Vitamin류 시험법 개선에 관한 연구
연
Validation of Vitamin E Analysis in Infant Formula by HPLC and
구
발표제목
Collaborative Study Result
성
과
발 표 자 Miyoung Baek, Jaewoo Park, Jisung Park, Sungok Song, Sunghwan Wee
활
발 표 처 한국식품과학회
발표시기
2012년 5월
용
집
Abstract :
The QIA official method of vitamin E isomers(α, β, γ, and δ tocopherol) in infant
formula is based on normal phase chromatography which the principle is outdated and
the difficulty of application in laboratories. Improving the applicability of the method
using commonly used reverse phase chromatography, the modified method is validated
by IUPAC guidelines for validation. Vitamin E isomers, α, β+γ, and δ were separated
by HPLC with a DAD detector using a C8 and C18 column. The vitamin E isomers
content in the SRM 1849 provided by NIST were analyzed within the range of certified
values with uncertainty. The LODs and LOQs for the vitamin E isomers ranged from
0.2 to 0.5mg/kg and 0.5 to 1.0mg/kg for C18 column and ranged from 0.1 to
0.2mg/kg and 0.3 to 1.0mg/kg for C8 column, respectively. Correlation coefficients for
the calibration curve were 0.998 to 0.999. The recovery of vitamins ranged from 92.6
? 97.7 %. The collaborative study, 10 laboratories participated, was performed by
AOAC guidelines and showed reproducibility as normally would be expected from the
HORRAT value between 0.5 and 1.5.
894
연
Validation of Vitamin E Analysis in Infant Formula by HPLC and
구
발표제목
Collaborative Study Result
성
과
발 표 자 Miyoung Baek, Jaewoo Park, Jisung Park, Sungok Song, Sunghwan Wee
활
발 표 처 한국식품과학회
발표시기
2012년 5월
용
집
Abstract :
The QIA official method of vitamin E isomers(α, β, γ, and δ tocopherol) in infant
formula is based on normal phase chromatography which the principle is outdated and
the difficulty of application in laboratories. Improving the applicability of the method
using commonly used reverse phase chromatography, the modified method is validated
by IUPAC guidelines for validation. Vitamin E isomers, α, β+γ, and δ were separated
by HPLC with a DAD detector using a C8 and C18 column. The vitamin E isomers
content in the SRM 1849 provided by NIST were analyzed within the range of certified
values with uncertainty. The LODs and LOQs for the vitamin E isomers ranged from
0.2 to 0.5mg/kg and 0.5 to 1.0mg/kg for C18 column and ranged from 0.1 to
0.2mg/kg and 0.3 to 1.0mg/kg for C8 column, respectively. Correlation coefficients for
the calibration curve were 0.998 to 0.999. The recovery of vitamins ranged from 92.6
? 97.7 %. The collaborative study, 10 laboratories participated, was performed by
AOAC guidelines and showed reproducibility as normally would be expected from the
HORRAT value between 0.5 and 1.5.
894
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