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학
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발표제목 False positive inducing parameters of LC-MS technique
발
표
발 표 자 권진욱, 박미정
게
발 표 처 ASMS(American Society for Mass Spectrometry
발표시기
2012년 5월
재
?
Abstract :
국
외
When performing multi component mixtures analysis by MRM or equivalent, it is
?
necessary to identify the target ions of specific m/z values with retention time.
Chemicals, having the same chemical structural backbone, for instance, sulfonamide
antimicrobials, possess the same fragment ion. Furthermore, stable isotope as an
internal standard has the almost same retention time with similar m/z that of native
standard. Although higher resolution of mass spectrometer are applied to analysis,
same or similar m/z ions, presenting the near or same retention time, could affect
among them to make artifactual signals, leading false positive result by atmospheric
pressure ionization(API). Identification of evidence to lead false positive result and
some parameters affecting these results by LC-MS application were investigated.
Data were acquired by ultra performance liquid chromatograph coupled TOF(Synapt G2
HDMS, Waters) and LC-MS/MS(Xevo TQ, Waters, Agilent 6430 Triple Quadrupole
LC/MS Systems). Clenbuterol, clenbuterol-d9, erythromycin, erythromycin-2-13C,
sulfamethazine, sulfamethazine-6-13C, and 14 sulfonamides including sulfadiazine,
sulfathiazole, were used to identify the abundance, interference, selectivity from the
single and mixture of native and stable isotope compounds with or without switching
the instrumental and separation condition.
Multiple Reaction Monitoring(MRM)/Selective Reaction Monitoring(SRM) by a triple
quadrupole mass spectrometer for quantitation and scanning by Time-of-Flight(TOF) for
screening of chemical residue in various matrices including food and environmental
samples, are commonly used mass spectrometry techniques. Advanced separation
technique with higher pressure resistance, so called ultra-performance, presents saving
the time of analysis. Exact mass were identified without any interferences, when single
native and stable isotope compounds were analyzed respectively under high resolution
of TOF scanning. But it is difficult to distinguish each of native and stable isotope
when mixtures were analyzed, and the increase of abundances of the selected specific
1301
학
술
발표제목 False positive inducing parameters of LC-MS technique
발
표
발 표 자 권진욱, 박미정
게
발 표 처 ASMS(American Society for Mass Spectrometry
발표시기
2012년 5월
재
?
Abstract :
국
외
When performing multi component mixtures analysis by MRM or equivalent, it is
?
necessary to identify the target ions of specific m/z values with retention time.
Chemicals, having the same chemical structural backbone, for instance, sulfonamide
antimicrobials, possess the same fragment ion. Furthermore, stable isotope as an
internal standard has the almost same retention time with similar m/z that of native
standard. Although higher resolution of mass spectrometer are applied to analysis,
same or similar m/z ions, presenting the near or same retention time, could affect
among them to make artifactual signals, leading false positive result by atmospheric
pressure ionization(API). Identification of evidence to lead false positive result and
some parameters affecting these results by LC-MS application were investigated.
Data were acquired by ultra performance liquid chromatograph coupled TOF(Synapt G2
HDMS, Waters) and LC-MS/MS(Xevo TQ, Waters, Agilent 6430 Triple Quadrupole
LC/MS Systems). Clenbuterol, clenbuterol-d9, erythromycin, erythromycin-2-13C,
sulfamethazine, sulfamethazine-6-13C, and 14 sulfonamides including sulfadiazine,
sulfathiazole, were used to identify the abundance, interference, selectivity from the
single and mixture of native and stable isotope compounds with or without switching
the instrumental and separation condition.
Multiple Reaction Monitoring(MRM)/Selective Reaction Monitoring(SRM) by a triple
quadrupole mass spectrometer for quantitation and scanning by Time-of-Flight(TOF) for
screening of chemical residue in various matrices including food and environmental
samples, are commonly used mass spectrometry techniques. Advanced separation
technique with higher pressure resistance, so called ultra-performance, presents saving
the time of analysis. Exact mass were identified without any interferences, when single
native and stable isotope compounds were analyzed respectively under high resolution
of TOF scanning. But it is difficult to distinguish each of native and stable isotope
when mixtures were analyzed, and the increase of abundances of the selected specific
1301
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