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Table 4. °¡±ÝƼǪ½º±Õ MLVA¿ë multiplex PCR ¹ÝÀÀ Á¶°Ç
¿¬
±¸
PCR ´Ü°è
¿Âµµ
½Ã°£
¼º
°ú
1 cycle
Predenaturation
94¡É
5ºÐ
È°
Denaturation
94¡É
30ÃÊ
¿ë
30 cycles
Annealing
62¡É
30ÃÊ
Áý
Extension
72¡É
1ºÐ
1 cycle
Final extension
72¡É
7ºÐ
Fig 2. SGTRÀ» µ¿½Ã¿¡ ÁõÆøÇϱâ À§ÇÑ Multiplex PCR. Lanes M, 100bp marker;
lane 1, biovar Gallinarum 287/91 (Reference); lane 2, biovar Gallinarum
SG06Q110; lane 3, biovar Gallinarum 9R; and lane 4, biovar Gallinarum SR2-N6.
? °¡±ÝƼǪ½º±Õ MLVA ºÐ¼®¿¡ µû¸¥ allele profile(type) °áÁ¤ ¹æ¹ý(Table 5 ¹× Table
6)
Table 5. PCR »ê¹° Å©±â(X) ÃøÁ¤(fragment analysis) ÈÄ VNTR ¹Ýº¹¼ö »êÁ¤
Tandem repeat
Allele number
SGTR1
(X-130)/8
SGTR2
(X-103)/45
SGTR3
(X-133)/7
SGTR4
(X-110)/39
380
¿¬
±¸
PCR ´Ü°è
¿Âµµ
½Ã°£
¼º
°ú
1 cycle
Predenaturation
94¡É
5ºÐ
È°
Denaturation
94¡É
30ÃÊ
¿ë
30 cycles
Annealing
62¡É
30ÃÊ
Áý
Extension
72¡É
1ºÐ
1 cycle
Final extension
72¡É
7ºÐ
Fig 2. SGTRÀ» µ¿½Ã¿¡ ÁõÆøÇϱâ À§ÇÑ Multiplex PCR. Lanes M, 100bp marker;
lane 1, biovar Gallinarum 287/91 (Reference); lane 2, biovar Gallinarum
SG06Q110; lane 3, biovar Gallinarum 9R; and lane 4, biovar Gallinarum SR2-N6.
? °¡±ÝƼǪ½º±Õ MLVA ºÐ¼®¿¡ µû¸¥ allele profile(type) °áÁ¤ ¹æ¹ý(Table 5 ¹× Table
6)
Table 5. PCR »ê¹° Å©±â(X) ÃøÁ¤(fragment analysis) ÈÄ VNTR ¹Ýº¹¼ö »êÁ¤
Tandem repeat
Allele number
SGTR1
(X-130)/8
SGTR2
(X-103)/45
SGTR3
(X-133)/7
SGTR4
(X-110)/39
380
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