총 1365페이지
1278페이지 본문시작
Table 2. 개발된 HA 및 NA 혈청형 동정용 rRT-PCR용 primer 염기서열
연
구
Subtype Lineage
a
Primer
Position
Accession
Size
Sequence
d
No. of mixed
Final primer
Dissociation
성
ID
on gene
c
number
(bp)
base positions
conc.
(°C)
과
H1
845
b
H1-887Feu
AF091309
305
GATGCTCCRGTYCAYAATT
3
83-84
Eu
2
847
b
H1-1191Reu
TTTGTGTGCTYTTYTGAT
2
활
849
b
H1-887Fam
AF091309
305
GATRCWCCAGTYCAYAAYT
5
83-84
Am
2
용
850
b
H1-1191Ram
TCTGYGTGCTYTTYTGRT
4
H2
479
H2-892F
L11127
343
AAMMCTTGAAAAYTGYGA
4
83-84
Eu + Am
2
집
682
b
H2-1234R
TTCAATYACRGASTTYACYTTG
5
H3
486
H3-919F
CY006031 376
GYATYACTCCWAATGGAAGC
3
83-84
Eu + Am
0.5
683
b
H3-1294R
ATYCTYCCTTCYACTTCDGM
5
H4
883
b
H4-896F
M25283
284
AGYAARTGYCAYACHGACA
5
84-85
Eu + Am
1
738
b
H4-1179R
GCTGCCTGRGTVGAYTTKAG
4
H5
491
H5-918F
U69277
261
CCARTRGGKGCKATAAAYTC
5
83-85
Eu + Am
2
820
b
H5-1178R
GTCTGCAGCRTAYCCACTYC
3
H6
511
H6-870F
CY005691 387
TCARAYYTDCCAATHGAGAA
5
83
Eu
2
855
b
H6-1256Reu
TCGTGRYCKACAGCYTCRA
5
511
H6-870F
CY005691 387
TCARAYYTDCCAATHGAGAA
5
83
Am
3
902
b
H6-1256Ram
TCRTGSTCKACWGCYTCRA
6
H7
815
b
H7-937Feu
M31689
241
ATMAATAGCAGRGCARTRGG
4
83
Eu
2
496
H7-1177R
GATCWATTGCHGAYTGRGTG
4
816
b
H7-937Fam
M31689
241
ATCAA CYCYA GRACW GTKGG
5
83
Am
2
496
H7-1177R
GATCWATTGCHGAYTGRGTG
4
H8
159
H8-854F
CY005970 376
GGCAGAATAATYCAAAATGA
1
83-84
Eu + Am
1
684
b
H8-1229R
TCATYTTRTCAACTATATTR
3
H9
781
b
H9-952F eu
CY005639 312
AGTAA RTAYG CRTTY GGRA
5
83-85
Eu
1
782
b
H9-1263R eu
TCRCT GAAYT CATGA TCRA
3
779
b
H9-919F am CY005639 312
AGCAA ATAYG CATTT GGGA
1
83-85
Am
2
780
b
H9-1229R am
TCAYT GAATT CATCA TYAA
2
H10
856
b
H10-935Feu CY014619 311
AAYYTRTCACCVAGRACAGT
5
83-85
Eu
2
501
H10-1245Reu
TCAGAYTCTATKGAYYCRAAC
5
825
b
H10-935Fam CY014619 311
AATCTTTCMCCRAGAACTGT
2
83-85
Am
2
826
b
H10-1245Ram
TCAGATTCTATGGATTCGAAC
H11
165
H11-858F
AY684895 410
GGAAATGGGAAAYTGTTCAG
1
83-84
Eu + Am
1
514
H11-1267R
AATTCRTGTTGHAYAGACTC
3
H12
543
H12-776F
CY005925 368
CARACWGTVAARATACARAC
5
84-86
Eu
1
857
b
H12-1143Reu
CCYGTTCCCTCRGARTTYTG
4
543
H12-776F
CY005925 368
CARACWGTVAARATACARAC
5
84-86
Am
1
903
b
H12-1143Ram
CCTGT CCCYT CYGCR TTTTG
3
H13
169
H13-856F
AY684887 337
ATTGARGARTATGGAAAAGG
2
84
Eu + Am
0.5
421
H13-1192R
GTYGAYTCTTTRTCTGCAGC
3
H14
171
H14-863F
CY014604 282
GAGCACAGTGCTTAAAAGTG
84
Eu + Am
0.5
422
H14-1144R
GCATTTTGRTGCCTRAATCCATACC
2
H15
515
H15-828F
L43916
273
CCGCTCTAATGCCCCWTCRG
2
84
Eu + Am
0.5
423
H15-1100R
TCGATGAATCCTGCAATTGC
H16
895
b
H16-834F
AY684888 371
GMTAY ATHAT TGAGA ARTACG
4
85
Eu + Am
1
897
b
H16-1204R
GTTGT TATYT CATYT ATKGC
3
N1
685
N1-54F
CY003931 245
TCARTCTGYATGRYAAYTGG
5
81-82
Eu + Am
0.5
469
N1-298R
GGRCARAGAGAKGAATTGCC
3
N2
470
N2-59F
CY005306 278
TYTCTMTAACYATTGCRWCARTAT
6
81-82
Eu + Am
2
695
N2-336R
GARTT GTCYT TRGAR AAVGG
5
N3
687
N3-79F
AY650272 287
GCCCTTCTYATYGGRRTKGGRAA
6
82-83
Eu + Am
2
688
N3-365R
ACTATDRCRTCYTTGTTYTC
5
N4
483
N4-55F
CY003986 236
AGTGYKAGYATTRTAYTRAC
6
81-82
Eu + Am
2
484
N4-290R
ARGTCTYTYCCACTRGARTA
5
N5
509
N5-115F
CY005693 178
GARTA ATATCAGYRACVAAAG
4
80-82
Eu + Am
1
87
N5-279R
GATACATYRCAGAGAGGTTC
2
N6
88
N6-57F
CY005464 264
AGGAATGACACTATCSGTAGTAAG
1
80-82
Eu + Am
1
89
N6-307R
GAYAGRATRTGCCATGAGTTYAC
4
N7
472
N7-53F
CY004347 261
TCWGGAGTGGCMATAGCACT
2
80-82
Eu + Am
0.5
473
N7-313R
CACKACCCAYCCTTCAACWTTG
3
N8
107
N8-93Feu
CY004056 137
CATRTVGTBAGYATYAYARTAAC
7
79-80
Eu
2
690
N8-209R
ACAYTRGYATTGTRCCATTG
4
788
b
N8-93Fam
CY004056 137
CAYATAGYTAGYATYACARTAAC
5
79-80
Am
2
690
N8-209R
ACAYTRGYATTGTRCCATTG
4
N9
691
N9-64Feu
CY004701 227
GTAATAGGCACRATYGCAGT
2
81-82
Eu
1
692
N9-290Reu
CCTTTRGTYARRTTATTGAA
4
790
b
N9-64Fam
CY004701 227
GTRATAGGCAYRATTGCAGT
3
81-82
Am
1
792
b
N9-290Ram
CCTYTRGTCARRTTRTTGAA
5
NP
551
NP-1200F
DQ064436 330
AGRTAYTGGGCYATAAGRAC
4
85
Eu + Am
1
806
NP-1529R
GCATTGTCTCCGAAGAAATAAG
a
Eu: for Eurasian lineage genes, Am: for American lineage genes
b
Primers updated in this study
c
Primers with eu or am or without indicate primers specific for Eurasian, American or common lineage genes, respectively.
d
Codes for mixed bases: B, C/G/T; D, A/G/T; H, A/T/C; K, G/T; M, A/C; R, A/G; S, C/G; V, A/C/G; W, A/T; Y, C/T.
384
연
구
Subtype Lineage
a
Primer
Position
Accession
Size
Sequence
d
No. of mixed
Final primer
Dissociation
성
ID
on gene
c
number
(bp)
base positions
conc.
(°C)
과
H1
845
b
H1-887Feu
AF091309
305
GATGCTCCRGTYCAYAATT
3
83-84
Eu
2
847
b
H1-1191Reu
TTTGTGTGCTYTTYTGAT
2
활
849
b
H1-887Fam
AF091309
305
GATRCWCCAGTYCAYAAYT
5
83-84
Am
2
용
850
b
H1-1191Ram
TCTGYGTGCTYTTYTGRT
4
H2
479
H2-892F
L11127
343
AAMMCTTGAAAAYTGYGA
4
83-84
Eu + Am
2
집
682
b
H2-1234R
TTCAATYACRGASTTYACYTTG
5
H3
486
H3-919F
CY006031 376
GYATYACTCCWAATGGAAGC
3
83-84
Eu + Am
0.5
683
b
H3-1294R
ATYCTYCCTTCYACTTCDGM
5
H4
883
b
H4-896F
M25283
284
AGYAARTGYCAYACHGACA
5
84-85
Eu + Am
1
738
b
H4-1179R
GCTGCCTGRGTVGAYTTKAG
4
H5
491
H5-918F
U69277
261
CCARTRGGKGCKATAAAYTC
5
83-85
Eu + Am
2
820
b
H5-1178R
GTCTGCAGCRTAYCCACTYC
3
H6
511
H6-870F
CY005691 387
TCARAYYTDCCAATHGAGAA
5
83
Eu
2
855
b
H6-1256Reu
TCGTGRYCKACAGCYTCRA
5
511
H6-870F
CY005691 387
TCARAYYTDCCAATHGAGAA
5
83
Am
3
902
b
H6-1256Ram
TCRTGSTCKACWGCYTCRA
6
H7
815
b
H7-937Feu
M31689
241
ATMAATAGCAGRGCARTRGG
4
83
Eu
2
496
H7-1177R
GATCWATTGCHGAYTGRGTG
4
816
b
H7-937Fam
M31689
241
ATCAA CYCYA GRACW GTKGG
5
83
Am
2
496
H7-1177R
GATCWATTGCHGAYTGRGTG
4
H8
159
H8-854F
CY005970 376
GGCAGAATAATYCAAAATGA
1
83-84
Eu + Am
1
684
b
H8-1229R
TCATYTTRTCAACTATATTR
3
H9
781
b
H9-952F eu
CY005639 312
AGTAA RTAYG CRTTY GGRA
5
83-85
Eu
1
782
b
H9-1263R eu
TCRCT GAAYT CATGA TCRA
3
779
b
H9-919F am CY005639 312
AGCAA ATAYG CATTT GGGA
1
83-85
Am
2
780
b
H9-1229R am
TCAYT GAATT CATCA TYAA
2
H10
856
b
H10-935Feu CY014619 311
AAYYTRTCACCVAGRACAGT
5
83-85
Eu
2
501
H10-1245Reu
TCAGAYTCTATKGAYYCRAAC
5
825
b
H10-935Fam CY014619 311
AATCTTTCMCCRAGAACTGT
2
83-85
Am
2
826
b
H10-1245Ram
TCAGATTCTATGGATTCGAAC
H11
165
H11-858F
AY684895 410
GGAAATGGGAAAYTGTTCAG
1
83-84
Eu + Am
1
514
H11-1267R
AATTCRTGTTGHAYAGACTC
3
H12
543
H12-776F
CY005925 368
CARACWGTVAARATACARAC
5
84-86
Eu
1
857
b
H12-1143Reu
CCYGTTCCCTCRGARTTYTG
4
543
H12-776F
CY005925 368
CARACWGTVAARATACARAC
5
84-86
Am
1
903
b
H12-1143Ram
CCTGT CCCYT CYGCR TTTTG
3
H13
169
H13-856F
AY684887 337
ATTGARGARTATGGAAAAGG
2
84
Eu + Am
0.5
421
H13-1192R
GTYGAYTCTTTRTCTGCAGC
3
H14
171
H14-863F
CY014604 282
GAGCACAGTGCTTAAAAGTG
84
Eu + Am
0.5
422
H14-1144R
GCATTTTGRTGCCTRAATCCATACC
2
H15
515
H15-828F
L43916
273
CCGCTCTAATGCCCCWTCRG
2
84
Eu + Am
0.5
423
H15-1100R
TCGATGAATCCTGCAATTGC
H16
895
b
H16-834F
AY684888 371
GMTAY ATHAT TGAGA ARTACG
4
85
Eu + Am
1
897
b
H16-1204R
GTTGT TATYT CATYT ATKGC
3
N1
685
N1-54F
CY003931 245
TCARTCTGYATGRYAAYTGG
5
81-82
Eu + Am
0.5
469
N1-298R
GGRCARAGAGAKGAATTGCC
3
N2
470
N2-59F
CY005306 278
TYTCTMTAACYATTGCRWCARTAT
6
81-82
Eu + Am
2
695
N2-336R
GARTT GTCYT TRGAR AAVGG
5
N3
687
N3-79F
AY650272 287
GCCCTTCTYATYGGRRTKGGRAA
6
82-83
Eu + Am
2
688
N3-365R
ACTATDRCRTCYTTGTTYTC
5
N4
483
N4-55F
CY003986 236
AGTGYKAGYATTRTAYTRAC
6
81-82
Eu + Am
2
484
N4-290R
ARGTCTYTYCCACTRGARTA
5
N5
509
N5-115F
CY005693 178
GARTA ATATCAGYRACVAAAG
4
80-82
Eu + Am
1
87
N5-279R
GATACATYRCAGAGAGGTTC
2
N6
88
N6-57F
CY005464 264
AGGAATGACACTATCSGTAGTAAG
1
80-82
Eu + Am
1
89
N6-307R
GAYAGRATRTGCCATGAGTTYAC
4
N7
472
N7-53F
CY004347 261
TCWGGAGTGGCMATAGCACT
2
80-82
Eu + Am
0.5
473
N7-313R
CACKACCCAYCCTTCAACWTTG
3
N8
107
N8-93Feu
CY004056 137
CATRTVGTBAGYATYAYARTAAC
7
79-80
Eu
2
690
N8-209R
ACAYTRGYATTGTRCCATTG
4
788
b
N8-93Fam
CY004056 137
CAYATAGYTAGYATYACARTAAC
5
79-80
Am
2
690
N8-209R
ACAYTRGYATTGTRCCATTG
4
N9
691
N9-64Feu
CY004701 227
GTAATAGGCACRATYGCAGT
2
81-82
Eu
1
692
N9-290Reu
CCTTTRGTYARRTTATTGAA
4
790
b
N9-64Fam
CY004701 227
GTRATAGGCAYRATTGCAGT
3
81-82
Am
1
792
b
N9-290Ram
CCTYTRGTCARRTTRTTGAA
5
NP
551
NP-1200F
DQ064436 330
AGRTAYTGGGCYATAAGRAC
4
85
Eu + Am
1
806
NP-1529R
GCATTGTCTCCGAAGAAATAAG
a
Eu: for Eurasian lineage genes, Am: for American lineage genes
b
Primers updated in this study
c
Primers with eu or am or without indicate primers specific for Eurasian, American or common lineage genes, respectively.
d
Codes for mixed bases: B, C/G/T; D, A/G/T; H, A/T/C; K, G/T; M, A/C; R, A/G; S, C/G; V, A/C/G; W, A/T; Y, C/T.
384
1278페이지 본문끝
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