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5. CPV-vp2
´Ü¹éÁúÀÇ
epitope
ÀÛ¼º
°¡
. 60bp
ÁßÈ
epitope
Ŭ·Î´×
CPV-vp2
´Ü¹éÁúÀÇ
5¡®
¸»´Ü¿¡ ÁßÈ´ÉÀÌ ÀÖ´Â ºÎÀ§
60bp
¸¦ ÁõÆøÇϱâ À§ÇØ
primer
A5'-AGAATCTAGTGATGGAGCAGT-3' primerB 5'-GGATCCCTAGTAGCTCTTTCAT-3' PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿©
¿Í
ÁõÆøÇÏ°í À̸¦
pGEMT-easy
º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ¿´´Ù
.
PrimerA
Neutrailizingepitope
N
C
CPVVP2
PrimerB
PrimerA: 5'-AGATCTAGTGATGGAGCAGT-3'(20mer)
PrimerB: 5'-GGATCCCTATGTAGCTCTTTCAT-3'(23mer)
75bp
³ª Ŭ·Î´×µÈ
.
epitope
ÀÇ
transfer vector
³»·Î »ðÀÔ
transfer vector pAcGHLT-c
·Î
60bp epitope
À» »ðÀÔÇÏ¿´°í ÀÌ º¤ÅÍ·Î ÀçÁ¶ÇÕµÈ º£
,
ŧ·Î¹ÙÀÌ·¯½º¸¦ ÀÛ¼ºÇÏ¿© À̷κÎÅÍ
epitope
À» »ý»êÇÒ ¿¹Á¤ÀÌ´Ù
.
T-vector cloning
MCS
60bp
AGTGATGGAGCAGTTCA¡¦...CAGAAATGAAAGAGCTACA
´Ü¹éÁúÀÇ
epitope
ÀÛ¼º
°¡
. 60bp
ÁßÈ
epitope
Ŭ·Î´×
CPV-vp2
´Ü¹éÁúÀÇ
5¡®
¸»´Ü¿¡ ÁßÈ´ÉÀÌ ÀÖ´Â ºÎÀ§
60bp
¸¦ ÁõÆøÇϱâ À§ÇØ
primer
A5'-AGAATCTAGTGATGGAGCAGT-3' primerB 5'-GGATCCCTAGTAGCTCTTTCAT-3' PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿©
¿Í
ÁõÆøÇÏ°í À̸¦
pGEMT-easy
º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ¿´´Ù
.
PrimerA
Neutrailizingepitope
N
C
CPVVP2
PrimerB
PrimerA: 5'-AGATCTAGTGATGGAGCAGT-3'(20mer)
PrimerB: 5'-GGATCCCTATGTAGCTCTTTCAT-3'(23mer)
75bp
³ª Ŭ·Î´×µÈ
.
epitope
ÀÇ
transfer vector
³»·Î »ðÀÔ
transfer vector pAcGHLT-c
·Î
60bp epitope
À» »ðÀÔÇÏ¿´°í ÀÌ º¤ÅÍ·Î ÀçÁ¶ÇÕµÈ º£
,
ŧ·Î¹ÙÀÌ·¯½º¸¦ ÀÛ¼ºÇÏ¿© À̷κÎÅÍ
epitope
À» »ý»êÇÒ ¿¹Á¤ÀÌ´Ù
.
T-vector cloning
MCS
60bp
AGTGATGGAGCAGTTCA¡¦...CAGAAATGAAAGAGCTACA
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