총 1326페이지
1144페이지 본문시작
수과원
표준기술
마우스 배아줄기세포 유래 조혈세포
조준형외
10. Rambhatla L et al. Generation of hepatocyte-like cells from human embryonic stem
(표준독성시험
완료(09.2.6)
활용
작출및유지배양기법
6인
cells. Cell transplant. 2003. 2, 1-11.
매뉴얼)
수과원
11. Rippon H. J. and Bishop A. E., Embryonic stem cells. Cell Prolif., 2004. 37, 23-34.
표준기술
마우스 배아줄기세포 유래 조혈세포
조준형외
(표준독성시험
완료(09.2.6)
12. Takamichi Ishii et al. In vitro differentiation and maturation of mouse embryonic
활용
성상확인기법
6인
매뉴얼)
stem cells into hepatocytes. Experiemental cell research. 2005. 309,. 68-77.
대한수의학회
조준형외
13. Wobus A. M. and Boheler K. R., Embryonic stem cells: Prospects for developmental
Establishment of mouse embryonic
학술발표
추계학술대회
완료(07.11.16)
8인
stem cell lines derived from 129SvJ
biology and cell therapy., Physiol. Rev. 2003. 85, 635-678.
구두발표
mice.
대한수의학회
조준형외
학술발표
춘계학술대회
완료(08.4.25)
Establishment of mouse embryonic
8인
포스터발표
carcinoma cell lines.
대한수의학회
Ⅹ. 영문초록
신효숙외
학술발표
추계학술대회
완료(08.9.25)
Comparative study for the formation
8인
포스터발표
Title
Establishment of Animal Stem Cells and Differentiated Cells
of murine embryoid body.
대한수의학회
(1st subtitle)
(Establishment, Characterization and maintenance of Animal Stem Cells)
이영주외
Invitro defferentiation of mouse
학술발표
추계학술대회
완료(08.9.25)
Joon-Hyoung Cho*, Sang-Hee Jeong, Hyo-Shuk Shin, Eun-Joo Kim, Jung-Hee
7인
embryonic stem cell-derived hepatic
포스터발표
Researcher
Jang, Byoung-Kook Choi, Jeong-Woo Kang and Gab-Soo Chung
progenitor cells.
- Abstract -
Introduction : Embryonic stem (ES) cells are derived from intracellular mass of blastocysts that
are totipotent to differentiate intoall types of cells in body and capable of unlimited and
Ⅶ. 참고문헌
undifferentiatedproliferation in appropriately controlled system. ES cells are regarded as a core
1. Buehr M., The primodial gem cells of mammals: some current perspectives., Exp.
resource for variable applications in biotechnology. Though there have been introduced several
Cell. Res. 1997. 232, 194-207
mice stem cell lines commercially used in bioscience area, the fundamental key technologies for
2. Evans M. J. and Kaufman M. H., Establishment in culture of pluripotential cells from
stem cell including isolation, culture, maintenance and characterization are essentially required for
mouse embryos., Nature. 1981. 292, 154-156.
making progress in stem cell research. Here we report the establishment of ES cell lines derived
3. Hayashi Y. Furue MK. Okamoto T. Ohnuma K. Myoishi Y. Fukuhara Y. Abe Y.
from 129/SvJ mice.
Sata JD. and Ashashima M., Integrins regulate mouse mebryonic stem cell
Materials and Methods:
self-renewal., Stem Cells. 2007. 25(12), 3005-3015
1. Isolation of blastosyst : 129/SvJ mice were maintained at 21~23℃in a specific pathogens-free
4. Hiroshi K. Tetsuya I. Mikiko K. Katsunori S and Yoshifumi A., A simple method for
animal room facility. Mice were mated overnight from 8 p.m. and confirmed the pregnancy
forming embryoid body from mouse embryonic stem cells., J. Biosci. Bioeng. 2003.
with the indication of vaginal plug the next morning. The day when the plug was observed
96(4), 409-411.
was allotted to be the first day of pregnancy (Day 1). In 3.5 days, pregnant mice were
5. Illmensee K. and Mintz B., Totipotency and normal differentiation of single
teratocarcinoma cells cloned by injection into blastocysts., Proc. Natl. Acad. Sci. 1976.
sacrificed and uterine was flushed out to obtain blastocysts.
73(2), 549-553.
2. Preparation of feeder layers : Mouse embryonic fibroblasts(3T3) were treated with mytomicin
6. Martin G. H., Isolation of a pluripotent cell line from early mouse embryos cultured
C (10ug/ml) to arrest the growth for 4 hours before MES cells loading.
in medium conditioned by teratocarcinoma stem cells., Proc. Natl. Acad. Sci. 1981.
3. Cell culturecondition : Dulbecco's modified Eagle's medium with high glucose (DMEM)
78(12), 7634-7638.
supplemented with 15% fetal bovine serum, 100unit/ml penicillin-100ug/ml streptomycin, 1%
7. National Institute of Health., Department of Health and Human Service., Stem Cells:
2-mercaptoethanol solution,1% neucleosides, 1% non-essential amino acid and 1,000 unit/ml of
Scientific Progress and Future Research Directions. 2001.
leukemia inhibitory factor (LIF) was used for isolation and maintenance of mouse ES (MES)
8. National Institute of Health., Department of Health and Human Service., Stem Cell
cells. MES cells were cultured on feeder layer in 37℃ 95% air/5% CO2condition and
Information: Stem Cell Basics. 2005.
subcultured every two days with medium change every day.
9. Qing-Jun Zhou, Li-Xin Xiang. In vitro differentiation of Hepatic progenitor cells from
4. Characterization of MES cells : The confirmation of the cultured cells as MES cell was
nouse embryonic stem cells induced by sodium butyrate. Journal of cellular
performed by the expression or non-expression of alkaline phosphatase(ALP), surface antigens
biochemistry. 2007. 100, 29-42.
(SSEA-1, SSEA-4, TRA-1-60), transcription factors (Nanog, Oct-4, Sox-2) and telomerase.
표준기술
마우스 배아줄기세포 유래 조혈세포
조준형외
10. Rambhatla L et al. Generation of hepatocyte-like cells from human embryonic stem
(표준독성시험
완료(09.2.6)
활용
작출및유지배양기법
6인
cells. Cell transplant. 2003. 2, 1-11.
매뉴얼)
수과원
11. Rippon H. J. and Bishop A. E., Embryonic stem cells. Cell Prolif., 2004. 37, 23-34.
표준기술
마우스 배아줄기세포 유래 조혈세포
조준형외
(표준독성시험
완료(09.2.6)
12. Takamichi Ishii et al. In vitro differentiation and maturation of mouse embryonic
활용
성상확인기법
6인
매뉴얼)
stem cells into hepatocytes. Experiemental cell research. 2005. 309,. 68-77.
대한수의학회
조준형외
13. Wobus A. M. and Boheler K. R., Embryonic stem cells: Prospects for developmental
Establishment of mouse embryonic
학술발표
추계학술대회
완료(07.11.16)
8인
stem cell lines derived from 129SvJ
biology and cell therapy., Physiol. Rev. 2003. 85, 635-678.
구두발표
mice.
대한수의학회
조준형외
학술발표
춘계학술대회
완료(08.4.25)
Establishment of mouse embryonic
8인
포스터발표
carcinoma cell lines.
대한수의학회
Ⅹ. 영문초록
신효숙외
학술발표
추계학술대회
완료(08.9.25)
Comparative study for the formation
8인
포스터발표
Title
Establishment of Animal Stem Cells and Differentiated Cells
of murine embryoid body.
대한수의학회
(1st subtitle)
(Establishment, Characterization and maintenance of Animal Stem Cells)
이영주외
Invitro defferentiation of mouse
학술발표
추계학술대회
완료(08.9.25)
Joon-Hyoung Cho*, Sang-Hee Jeong, Hyo-Shuk Shin, Eun-Joo Kim, Jung-Hee
7인
embryonic stem cell-derived hepatic
포스터발표
Researcher
Jang, Byoung-Kook Choi, Jeong-Woo Kang and Gab-Soo Chung
progenitor cells.
- Abstract -
Introduction : Embryonic stem (ES) cells are derived from intracellular mass of blastocysts that
are totipotent to differentiate intoall types of cells in body and capable of unlimited and
Ⅶ. 참고문헌
undifferentiatedproliferation in appropriately controlled system. ES cells are regarded as a core
1. Buehr M., The primodial gem cells of mammals: some current perspectives., Exp.
resource for variable applications in biotechnology. Though there have been introduced several
Cell. Res. 1997. 232, 194-207
mice stem cell lines commercially used in bioscience area, the fundamental key technologies for
2. Evans M. J. and Kaufman M. H., Establishment in culture of pluripotential cells from
stem cell including isolation, culture, maintenance and characterization are essentially required for
mouse embryos., Nature. 1981. 292, 154-156.
making progress in stem cell research. Here we report the establishment of ES cell lines derived
3. Hayashi Y. Furue MK. Okamoto T. Ohnuma K. Myoishi Y. Fukuhara Y. Abe Y.
from 129/SvJ mice.
Sata JD. and Ashashima M., Integrins regulate mouse mebryonic stem cell
Materials and Methods:
self-renewal., Stem Cells. 2007. 25(12), 3005-3015
1. Isolation of blastosyst : 129/SvJ mice were maintained at 21~23℃in a specific pathogens-free
4. Hiroshi K. Tetsuya I. Mikiko K. Katsunori S and Yoshifumi A., A simple method for
animal room facility. Mice were mated overnight from 8 p.m. and confirmed the pregnancy
forming embryoid body from mouse embryonic stem cells., J. Biosci. Bioeng. 2003.
with the indication of vaginal plug the next morning. The day when the plug was observed
96(4), 409-411.
was allotted to be the first day of pregnancy (Day 1). In 3.5 days, pregnant mice were
5. Illmensee K. and Mintz B., Totipotency and normal differentiation of single
teratocarcinoma cells cloned by injection into blastocysts., Proc. Natl. Acad. Sci. 1976.
sacrificed and uterine was flushed out to obtain blastocysts.
73(2), 549-553.
2. Preparation of feeder layers : Mouse embryonic fibroblasts(3T3) were treated with mytomicin
6. Martin G. H., Isolation of a pluripotent cell line from early mouse embryos cultured
C (10ug/ml) to arrest the growth for 4 hours before MES cells loading.
in medium conditioned by teratocarcinoma stem cells., Proc. Natl. Acad. Sci. 1981.
3. Cell culturecondition : Dulbecco's modified Eagle's medium with high glucose (DMEM)
78(12), 7634-7638.
supplemented with 15% fetal bovine serum, 100unit/ml penicillin-100ug/ml streptomycin, 1%
7. National Institute of Health., Department of Health and Human Service., Stem Cells:
2-mercaptoethanol solution,1% neucleosides, 1% non-essential amino acid and 1,000 unit/ml of
Scientific Progress and Future Research Directions. 2001.
leukemia inhibitory factor (LIF) was used for isolation and maintenance of mouse ES (MES)
8. National Institute of Health., Department of Health and Human Service., Stem Cell
cells. MES cells were cultured on feeder layer in 37℃ 95% air/5% CO2condition and
Information: Stem Cell Basics. 2005.
subcultured every two days with medium change every day.
9. Qing-Jun Zhou, Li-Xin Xiang. In vitro differentiation of Hepatic progenitor cells from
4. Characterization of MES cells : The confirmation of the cultured cells as MES cell was
nouse embryonic stem cells induced by sodium butyrate. Journal of cellular
performed by the expression or non-expression of alkaline phosphatase(ALP), surface antigens
biochemistry. 2007. 100, 29-42.
(SSEA-1, SSEA-4, TRA-1-60), transcription factors (Nanog, Oct-4, Sox-2) and telomerase.
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