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Colletotrichum nymphaeae에 대한 첫 번째 보고입니다.
3. 원 문
First Report of Colletotrichum fioriniae and C. nymphaeae as Causal Agents of Anthracnose on
Walnut in Italy
L. Luongo, M. Galli, I. Garaguso, M. Petrucci, and S. Vitale
English walnut (Juglans regia L.) is a species grown for either high quality wood or fruit
production. In Italy walnut cultivation occupies an area of about 4,600 ha
(http://www.fao.org/faostat). In 2019 to 2020, walnut fruits (cv. Lara) with anthracnose
symptoms were collected from walnut orchards in the Province of Venice (Northern Italy).
Affected fruits showed necrotic and circular lesions with acervuli in the center, causing the
complete mummification of the fruit as described by Da Lio et al. (2018). Orange conidial
masses appeared under wet conditions. The fungus was isolated from necrotic tissues, and
conidial masses were put on potato dextrose agar (PDA) medium. Plates were incubated at 25°C
for 5 to 7 days. The colonies were white to pink on the upper side and pink with black spots
on the reverse. Acervuli formed and produced conidial masses on PDA after 6 days. Culture
and conidial morphology were in concordance with published descriptions of C. acutatum
sensu lato (Damm et al. 2012). To confirm the identity, internal transcribed spacers (ITS),
glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and beta-tubulin (TUB2)
genes were amplified and sequenced using the primer pairs ITS1/ITS4 (White et al. 1990),
GDF1/GDR1 (Guerber et al. 2003), ACT512F/ACT783R, and BT2Fd/BT4R (Da Lio et al. 2018).
The isolates belonged to two different species of Colletotrichum acutatum complex: C. fioriniae
(Marcelino & Gouli) and C. nymphaeae (Pass). Sequences of two representative isolates, C.
fioriniae CREADC-F2317 and C. nymphaeae CREADC-F2372, were deposited in GenBank with
accession numbers MZ153170 and MZ191794 (ITS), MZ203522 and MZ224013 (GAPDH), MZ203521
and MZ224012 (ACT), and MZ203523 and MZ224014 (TUB2). For all the genes, isolates had a
100% similarity to multiple C. fioriniae and C. nymphaeae accessions, respectively. Maximum
likelihood trees based on concatenated sequences of the four genes were constructed using
MEGA 6.0 (Tamura et al. 2013). The phylogenetic analysis grouped the isolates in the C.
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3. 원 문
First Report of Colletotrichum fioriniae and C. nymphaeae as Causal Agents of Anthracnose on
Walnut in Italy
L. Luongo, M. Galli, I. Garaguso, M. Petrucci, and S. Vitale
English walnut (Juglans regia L.) is a species grown for either high quality wood or fruit
production. In Italy walnut cultivation occupies an area of about 4,600 ha
(http://www.fao.org/faostat). In 2019 to 2020, walnut fruits (cv. Lara) with anthracnose
symptoms were collected from walnut orchards in the Province of Venice (Northern Italy).
Affected fruits showed necrotic and circular lesions with acervuli in the center, causing the
complete mummification of the fruit as described by Da Lio et al. (2018). Orange conidial
masses appeared under wet conditions. The fungus was isolated from necrotic tissues, and
conidial masses were put on potato dextrose agar (PDA) medium. Plates were incubated at 25°C
for 5 to 7 days. The colonies were white to pink on the upper side and pink with black spots
on the reverse. Acervuli formed and produced conidial masses on PDA after 6 days. Culture
and conidial morphology were in concordance with published descriptions of C. acutatum
sensu lato (Damm et al. 2012). To confirm the identity, internal transcribed spacers (ITS),
glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and beta-tubulin (TUB2)
genes were amplified and sequenced using the primer pairs ITS1/ITS4 (White et al. 1990),
GDF1/GDR1 (Guerber et al. 2003), ACT512F/ACT783R, and BT2Fd/BT4R (Da Lio et al. 2018).
The isolates belonged to two different species of Colletotrichum acutatum complex: C. fioriniae
(Marcelino & Gouli) and C. nymphaeae (Pass). Sequences of two representative isolates, C.
fioriniae CREADC-F2317 and C. nymphaeae CREADC-F2372, were deposited in GenBank with
accession numbers MZ153170 and MZ191794 (ITS), MZ203522 and MZ224013 (GAPDH), MZ203521
and MZ224012 (ACT), and MZ203523 and MZ224014 (TUB2). For all the genes, isolates had a
100% similarity to multiple C. fioriniae and C. nymphaeae accessions, respectively. Maximum
likelihood trees based on concatenated sequences of the four genes were constructed using
MEGA 6.0 (Tamura et al. 2013). The phylogenetic analysis grouped the isolates in the C.
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