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water. The inoculated branches were placed in sterile plastic containers to maintain high
humidity and incubated for 10 days at 28°C. After 7 days, irregular lesions and fungal growth
were observed at the site of inoculation. The affected area gradually increased in size with
similar symptomatology to that described above. Reisolation of the pathogen and identification
based on morphological features and sequencing confirmed the presence of A. alternata. To
our knowledge, this is the first report of A. alternata affecting branches of thuja in
Kazakhstan. Thuja is a rare species for this region; its cost and care are expensive. This
study will allow timely diagnosis of the disease caused by Alternaria spp. It is necessary to
develop preventive measures and a protocol for the treatment of thuja with fungal infection.
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