총 622페이지

Efficient Elimination of Actinidia Chlorotic Ringspot-Associated Virus from Infected Kiwifruit
Shoots Cultured In Vitro
Yicheng Wang, Jiaxiu Sun, Jingke Wang, Shrestha Sujata, Qianru Huang, Caiting Hou, Yunfeng
Wu, and Lei Zhao
Abstract
In this study, methods of Actinidia chlorotic ringspot-associated virus (AcCRaV) elimination by
shoot tip culture, thermotherapy followed by shoot tip culture, and chemotherapy followed by
shoot tip culture were explored. The results showed that the AcCRaV elimination rate was
23.3% when the secondary shoot tip culture method was used and when the shoot tip length
was less than 0.5 mm. The AcCRaV elimination rate was 100% when thermotherapy (36°C [day]
and 32°C [night]) was applied for 20 days followed by shoot tip culture (shoot tip length less
than 1.0 mm). When shoot segments were treated with ribavirin at 15 μg/ml for 2 months
followed by shoot tip culture, the elimination rate of AcCRaV was 100% (shoot tip length less
than 1.0 mm). When shoot segments were treated with ribavirin at 25 μg/ml for 2 months
followed by shoot tip culture, the elimination rate of AcCRaV was 100% (shoot tip length less
than 1.5 mm). This is the first report on kiwifruit virus elimination methods.
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