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cv. Draper and Duke. A small piece of the bark tissue was removed with a sterile-bladed
scalpel to expose the cambium. Mycelium plugs (5 mm diameter) from 10-day-old cultures of
the strains CVG1130 and CVG1131 were placed with the mycelium in contact with the internal
plant tissues. Three plants were used for each isolate and the inoculation points were sealed
with Parafilm. Three plants treated with sterile PDA plugs were used as a control. Plants were
placed in a growth chamber at 25°C for 3 weeks. After this period, symptoms similar to those
observed in the field appeared on the inoculated plants, while control plants remained healthy.
A fungus with the same morphological characteristics of D. eres was reisolated from
inoculated plants and identified by sequencing the tub2 gene to confirm Koch’s postulates.
Diaporthe eres was previously reported on different Vaccinium spp. in Chile, Lithuania, the
Netherlands, and the United States (Farr and Rossman 2022). To our knowledge, this is the
first report of D. eres associated with stem blight and dieback of highbush blueberry in Italy.
Duke and Draper are among the most cultivated blueberry cultivars in Piedmont, where the
spread of D. eres could represent a serious threat.
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scalpel to expose the cambium. Mycelium plugs (5 mm diameter) from 10-day-old cultures of
the strains CVG1130 and CVG1131 were placed with the mycelium in contact with the internal
plant tissues. Three plants were used for each isolate and the inoculation points were sealed
with Parafilm. Three plants treated with sterile PDA plugs were used as a control. Plants were
placed in a growth chamber at 25°C for 3 weeks. After this period, symptoms similar to those
observed in the field appeared on the inoculated plants, while control plants remained healthy.
A fungus with the same morphological characteristics of D. eres was reisolated from
inoculated plants and identified by sequencing the tub2 gene to confirm Koch’s postulates.
Diaporthe eres was previously reported on different Vaccinium spp. in Chile, Lithuania, the
Netherlands, and the United States (Farr and Rossman 2022). To our knowledge, this is the
first report of D. eres associated with stem blight and dieback of highbush blueberry in Italy.
Duke and Draper are among the most cultivated blueberry cultivars in Piedmont, where the
spread of D. eres could represent a serious threat.
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