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segments L, M, and S, respectively. The presence of TSWV was confirmed in the
high-throughput sequencing (HTS) sample using an RT-PCR assay (primers L1 and L2)
targeting the L segment of TSWV (Mumford et al. 1994). In July 2022, additional leaf samples
displaying symptoms of chlorotic mottling, streaking, and ringspots were collected from 31
symptomatic and 3 asymptomatic agapanthus plants in public gardens in Stellenbosch, South
Africa. Using the abovementioned RT-PCR assay, 13 of the symptomatic samples tested positive
for TSWV. All six plants displaying ring spot symptoms were infected with TSWV. However,
plants that displayed yellow streaking (five samples) and chlorotic mottling (two samples) were
also positive for TSWV, which could be because of the presence of other viruses, plant growth
stage, infection time, or just variable symptom expression in a single host species as reported
previously (Sherwood et al. 2003). The 275-bp RT-PCR amplicons of the HTS sample and three
additional positive samples were validated with bidirectional Sanger sequencing (CAF) and had
96% identity to the accession KY250488. The pairwise nt identity between amplicons was 98.55
to 100%. This is the first report of TSWV infecting agapanthus plants in South Africa. This
study contributes information toward the distribution and incidence of TSWV and highlights
the need for nurseries to screen plant material before propagation.
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